Pro-Pol polyprotein (pol) Recombinant Protein | pol recombinant protein
Recombinant Feline foamy virus Pro-Pol polyprotein (pol)
NCBI and Uniprot Product Information
Uniprot Description
The aspartyl protease activity mediates proteolytic cleavages of Gag and Pol polyproteins. The reverse transcriptase (RT) activity converts the viral RNA genome into dsDNA in the cytoplasm, shortly after virus entry into the cell (early reverse transcription) or after proviral DNA transcription (late reverse transcription). RT consists of a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3' to 5' endonucleasic mode. Conversion of viral genomic RNA into dsDNA requires many steps. A tRNA-Lys1,2 binds to the primer-binding site (PBS) situated at the 5'-end of the viral RNA. RT uses the 3' end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis. The reading proceeds through the U5 region and ends after the repeated (R) region which is present at both ends of viral RNA. The portion of the RNA-DNA heteroduplex is digested by the RNase H, resulting in a ssDNA product attached to the tRNA primer. This ssDNA/tRNA hybridizes with the identical R region situated at the 3' end of viral RNA. This template exchange, known as minus-strand DNA strong stop transfer, can be either intra- or intermolecular. RT uses the 3' end of this newly synthesized short ssDNA to perform the RNA-dependent minus-strand DNA synthesis of the whole template. RNase H digests the RNA template except for a polypurine tract (PPT) situated at the 5'-end and near the center of the genome. It is not clear if both polymerase and RNase H activities are simultaneous. RNase H probably can proceed both in a polymerase-dependent (RNA cut into small fragments by the same RT performing DNA synthesis) and a polymerase-independent mode (cleavage of remaining RNA fragments by free RTs). Secondly, RT performs DNA-directed plus-strand DNA synthesis using the PPT that has not been removed by RNase H as primer. PPT and tRNA primers are then removed by RNase H. The 3' and 5' ssDNA PBS regions hybridize to form a circular dsDNA intermediate. Strand displacement synthesis by RT to the PBS and PPT ends produces a blunt ended, linear dsDNA copy of the viral genome that includes long terminal repeats (LTRs) at both ends ().
Research Articles on pol
Similar Products
Product Notes
The pol pol (Catalog #AAA1003157) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-1156, Full length protein. The amino acid sequence is listed below: MDLLKPLTVE RKGVKIKGYW DSQADITCVP KDLLQGEEPV RQQNVTTIHG TQEGDVYYVN LKIDGRRINT EVIGTTLDYA IITPGDVPWI LKKPLELTIK LDLEEQQGTL LNNSILSKKG KEELKQLFEK YSALWQSWEN QVGHRRIRPH KIATGTVKPT PQKQYHINPK AKPDIQIVIN DLLKQGVLIQ KESTMNTPVY PVPKPNGRWR MVLDYRAVNK VTPLIAVQNQ HSYGILGSLF KGRYKTTIDL SNGFWAHPIV PEDYWITAFT WQGKQYCWTV LPQGFLNSPG LFTGDVVDLL QGIPNVEVYV DDVYISHDSE KEHLEYLDIL FNRLKEAGYI ISLKKSNIAN SIVDFLGFQI TNEGRGLTDT FKEKLENITA PTTLKQLQSI LGLLNFARNF IPDFTELIAP LYALIPKSTK NYVPWQIEHS TTLETLITKL NGAEYLQGRK GDKTLIMKVN ASYTTGYIRY YNEGEKKPIS YVSIVFSKTE LKFTELEKLL TTVHKGLLKA LDLSMGQNIH VYSPIVSMQN IQKTPQTAKK ALASRWLSWL SYLEDPRIRF FYDPQMPALK DLPAVDTGKD NKKHPSNFQH IFYTDGSAIT SPTKEGHLNA GMGIVYFINK DGNLQKQQEW SISLGNHTAQ FAEIAAFEFA LKKCLPLGGN ILVVTDSNYV AKAYNEELDV WASNGFVNNR KKPLKHISKW KSVADLKRLR PDVVVTHEPG HQKLDSSPHA YGNNLADQLA TQASFKVHMT KNPKLDIEQI KAIQACQNNE RLPVGYPKQY TYELQNNKCM VLRKDGWREI PPSRERYKLI KEAHNISHAG REAVLLKIQE NYWWPKMKKD ISSFLSTCNV CKMVNPLNLK PISPQAIVHP TKPFDKFYMD YIGPLPPSEG YVHVLVVVDA ATGFTWLYPT KAQTSKATIK VLNHLTGLAI PKVLHSDQGS AFTSEEFAQW AKERNIQLEF STPYHPQSSG KVERKNSEIK KLLTKLLVGR PLKWYNLISS VQLALNNTHV VSTKYTPHQL MFGIDCNLPF ANKDTLDWTR EEELALLQEI RESLQHPVQP PTCSGWSPYV GQLVQERVYR PSQLRPKWRK PTKVLEILNP RTVIIVDHLG QRKSVSIDNL KPTAHQHNGT RTCDDPEGMD GMECSQTTTE TSVDSS. It is sometimes possible for the material contained within the vial of "Pro-Pol polyprotein (pol), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
