Podxl recombinant protein

Podxl, 22-404aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206271

• Gene Names: Podxl; PC; Ly102; Pclp1; PCLP-1; Podxl1; AW121214
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Podxl; 22-404aa; Mouse; His tag; Baculovirus; Podocalyxin; AW121214; Ly102; PC; PCLP-1; Pclp1; Podxl recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: HNGNETSTSA IKSSTVQSHQ SATTSTEVTT GHPVASTLAS TQPSNPTPFT TSTQSPSMPT STPNPTSNQS GGNLTSSVSE VDKTKTSSPS STAFTSSSGQ TASSGGKSGD SFTTAPTTTL GLINVSSQPT DLNTTSKLLS TPTTDNTTSP QQPVDSSPST ASHPVGQHTP AAVPSSSGST PSTDNSTLTW KPTTHKPLGT SEATQPLTSQ TPGITTLPVS TLQQSMASTV GTTTEEFTHL ISNGTPVAPP GPSTPSPIWA FGNYQLNCEP PIRPDEELLI LNLTRASLCE RSPLDEKEKL VELLCHSVKA SFKPAEDLCT LHVAPILDNQ AVAVKRIIIE TKLSPKAVYE LLKDRWDDLT EAGVSDMKLG KEGPPEVNED RFSLEHHHHH H
• Sequence Length: 503

• Applicable Applications for Podxl recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Podxl recombinant protein

PODXL, also known as Podocalyxin, is a heavily glycosylated transmembrane sialoprotein in the CD34 and endoglycan family. This protein involved in the regulation of both adhesion and cell morphology and cancer progression. It has to function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Also, it acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Recombinant mouse PODXL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Podxl recombinant protein

Signal Transduction; Cytoskeleton

References

Vitureira N., et al. (2010) PLoS ONE 8:e12003.; Nielsen JS., et al. (2007) PLoS ONE 2:E237.

NCBI and Uniprot Product Information

• NCBI GI #: 34328219
• NCBI GeneID: 27205
• NCBI Accession #: NP_038751
• NCBI GenBank Nucleotide #: NM_013723.3
• UniProt Accession #: Q9R0M4; Q9ESZ1
• Molecular Weight: 41.0kDa (391aa) 70-100KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: podocalyxin
• NCBI Official Synonym Full Names: podocalyxin-like
• NCBI Official Symbol: Podxl
• NCBI Official Synonym Symbols: PC; Ly102; Pclp1; PCLP-1; Podxl1; AW121214
• NCBI Protein Information: podocalyxin
• UniProt Protein Name: Podocalyxin
• Protein Family: Podocalyxin
• UniProt Gene Name: Podxl
• UniProt Synonym Gene Names: Pclp1; PC; PCLP-1

Uniprot Description

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.