PNLIP recombinant protein

PNLIP Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121467

• Gene Names: PNLIP; PL; PTL; PNLIPD
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PNLIP; PNLIP Protein; Human; Recombinant (His Tag); Human PNLIP Protein (His Tag); PL Protein; PNLIPD Protein; PTL Protein; pancreatic lipase; PNLIP recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys465

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PNLIP (P16233)(Met1-Cys465) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PNLIP recombinant protein

Background: PNLIP is an enzyme that belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

References

Hegele RA, et al. (2001) Polymorphisms in PNLIP, encoding pancreatic lipase, and associations with metabolic traits. J Hum Genet. 46(6):320-4. Thomas A, et al. (2005) Role of the lid hydrophobicity pattern in pancreatic lipase activity. J Biol Chem. 280(48):40074-83. Colin DY, et al. (2008) Exploring the active site cavity of human pancreatic lipase. Biochem Biophys Res Commun. 370(3):394-8.

NCBI and Uniprot Product Information

• NCBI GI #: 1304379
• NCBI GeneID: 5406
• UniProt Accession #: P16233; Q5VSQ2
• Molecular Weight: 51,157 Da
• NCBI Official Full Name: pancreatic lipase
• NCBI Official Synonym Full Names: pancreatic lipase
• NCBI Official Symbol: PNLIP
• NCBI Official Synonym Symbols: PL; PTL; PNLIPD
• NCBI Protein Information: pancreatic triacylglycerol lipase; triacylglycerol acylhydrolase
• UniProt Protein Name: Pancreatic triacylglycerol lipase
• Protein Family: Pancreatic triacylglycerol lipase
• UniProt Gene Name: PNLIP
• UniProt Synonym Gene Names: PL; PTL
• UniProt Entry Name: LIPP_HUMAN

NCBI Description

This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Protein type: EC 3.1.1.3; Lipid Metabolism - glycerolipid; Secreted; Secreted, signal peptide; Hydrolase

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: extracellular region

Molecular Function: triacylglycerol lipase activity; metal ion binding

Biological Process: phototransduction, visible light; cholesterol absorption; retinoid metabolic process; lipid catabolic process; lipid digestion

Disease: Pancreatic Lipase Deficiency