Mouse anti-Human CD38 Monoclonal Antibody | anti-CD38 antibody

Mouse anti-Human CD38, FITC Conjugated mAb

Cat. Num. AAA8501154

• Gene Names: CD38; ADPRC1; ADPRC 1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: CD38; Monoclonal Antibody; Mouse anti-Human CD38; FITC Conjugated mAb; anti-CD38 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4AHIT2
• Form/Format: Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
• Sequence Length: 300

• Applicable Applications for anti-CD38 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; ; 2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light; ; 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); ; 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).; [PBS wash buffer: PBS +1% FBS +0.1% NaN3]; [Cell fixation: 2% formaldehyde solution]
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Testing Data

(PHA and ConA activated lymphocytes analyzed with FITC CD38 mAb)

Related Product Information for anti-CD38 antibody

4AHIT2 reacts with CD38 antigen, a 45 kDa integral membrane glycoprotein expressed on all pre-B cells, plasma cells, thymocytes, activated T cells, NK cells, monocyte/macrophages and dentritic cells. CD38 antigen is expressed 90% of CD34+ cells, but not on pluripotent stem cells. Coexpression of CD38 + and CD34+ indicates lineage commitment of those cells. CD38 antigen acts as an ectoenzyme capable of catalysing multipe reactions and play role on regulator of cell activation and proleferation depending on cellular enviroment. Application: Leukemia phenotyping and classification; monitoring of HIV-1 infection and progression.

References

1. Ling NR et al., eds. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York. 303-334. 2. Terstappen LWMM et al., (1992) Blood 79: 666. 3. Deaglio S et al., (2001) Leuk Res 25:1. 4. Ghia P et al., (2003) Blood 101:1262.

NCBI and Uniprot Product Information

• NCBI GI #: 1911103
• NCBI GeneID: 952
• Molecular Weight: 13,788 Da
• NCBI Official Full Name: CD38
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: ADPRC1; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• Protein Family: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1

NCBI Description

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.