PNLIP recombinant protein

Recombinant Human PNLIP Protein (His tag)

Cat. Num. AAA2545281

• Gene Names: PNLIP; PL; PTL; PNLIPD
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: PNLIP; Recombinant Human PNLIP Protein (His tag); PNLIP recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 465

• Application Notes: The recombinant human PNLIP consists of 460 amino acids and predicts a molecular mass of 51 KDa. It migrates as an approximately 51 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Lys 17
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PNLIP recombinant protein

Background: PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

Description: A DNA sequence encoding the human PNLIP (P16233) (Met1-Cys465) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 10835000
• NCBI GeneID: 5406
• NCBI Accession #: NP_000927.1
• NCBI GenBank Nucleotide #: NM_000936.3
• UniProt Accession #: P16233; Q5VSQ2
• Molecular Weight: 51,157 Da
• NCBI Official Full Name: pancreatic triacylglycerol lipase
• NCBI Official Synonym Full Names: pancreatic lipase
• NCBI Official Symbol: PNLIP
• NCBI Official Synonym Symbols: PL; PTL; PNLIPD
• NCBI Protein Information: pancreatic triacylglycerol lipase
• UniProt Protein Name: Pancreatic triacylglycerol lipase
• Protein Family: Pancreatic triacylglycerol lipase
• UniProt Gene Name: PNLIP
• UniProt Synonym Gene Names: PL; PTL; Pancreatic lipase

NCBI Description

This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea. [provided by RefSeq, Jul 2016]

Uniprot Description

PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Protein type: EC 3.1.1.3; Hydrolase; Lipid Metabolism - glycerolipid; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: extracellular region

Molecular Function: triacylglycerol lipase activity

Biological Process: lipid digestion; retinoid metabolic process

Disease: Pancreatic Lipase Deficiency