Rabbit anti-Human DMRT1 Polyclonal Antibody | anti-DMRT1 antibody

DMRT1 (Doublesex and mab-3 Related Transcription Factor 1, DM Domain Expressed in Testis Protein 1, DMT1) (Biotin)

Cat. Num. AAA6376372

• Gene Names: DMRT1; DMT1; CT154
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DMRT1; Polyclonal Antibody; DMRT1 (Doublesex and mab-3 Related Transcription Factor 1; DM Domain Expressed in Testis Protein 1; DMT1) (Biotin); anti-DMRT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DMRT1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DMRT1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DMRT1, aa1-373 (NP_068770.2).
• Immunogen Sequence: MPNDEAFSKPSTPSEAPHAPGVPPQGRAGGFGKASGALVGAASGSSAGGSSRGGGSGSGASDLGAGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCNLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENNGSNPCLMTECSGTSQPPPASVPTTAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYYSSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVGNPLGGSPVKNSLRGLPGPYVPGQTGNQWQMKNMENRHAMSSQYRMHSYYPPPSYLGQSVPQFFTFEDAPSYPEARASVFSPPSSQDSGLVSLSSSSPISNKSTKAVLECEPASEPSSFTVTPVIEEDE
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DMRT1 expression in transfected 293T cell line by DMRT1 polyclonal antibody. Lane 1: DMRT1 transfected lysate (39.5kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DMRT1 antibody

This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.

Product Categories/Family for anti-DMRT1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 62241026
• NCBI GeneID: 1761
• NCBI Accession #: NP_068770.2
• NCBI GenBank Nucleotide #: NM_021951.2
• UniProt Accession #: Q9Y5R6; Q6T1H8; Q6T1H9; Q8IW77; B2R913
• Molecular Weight: 18,325 Da
• NCBI Official Full Name: doublesex- and mab-3-related transcription factor 1
• NCBI Official Synonym Full Names: doublesex and mab-3 related transcription factor 1
• NCBI Official Symbol: DMRT1
• NCBI Official Synonym Symbols: DMT1; CT154
• NCBI Protein Information: doublesex- and mab-3-related transcription factor 1
• UniProt Protein Name: Doublesex- and mab-3-related transcription factor 1
• Protein Family: Doublesex and mab-3 related transcription factor
• UniProt Gene Name: DMRT1
• UniProt Synonym Gene Names: DMT1
• UniProt Entry Name: DMRT1_HUMAN

NCBI Description

This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. [provided by RefSeq, Jul 2008]

Uniprot Description

DMRT1: Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis. Defects in DMRT1 may be a cause of testicular germ cell tumor (TGCT). A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. Defects in DMRT1 may be a cause of 46,XY sex reversal type 4 (SRXY4). A condition characterized by male-to- female sex reversal in the presence of a normal 46,XY karyotype. Patients display complete or partial gonadal dysgenesis and a chromosome 9p deletion. Belongs to the DMRT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: cytoplasm; nucleus

Molecular Function: chromatin binding; metal ion binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity

Biological Process: cell morphogenesis; germ cell migration; male sex determination; male sex differentiation; negative regulation of meiosis; negative regulation of transcription from RNA polymerase II promoter; oocyte development; positive regulation of mitosis; positive regulation of transcription from RNA polymerase II promoter; Sertoli cell development; Sertoli cell differentiation; sex differentiation; spermatogenesis; transcription from RNA polymerase II promoter