SDS-Page
(Recombinant PLK3 (57-340) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 33.7 kDa Purity: >85%)
PLK3 recombinant protein
Recombinant PLK3 (57-340) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PLK3 (57-340) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 33.7 kDa Purity: >85%)
SDS-Page
(Recombinant PLK3 (57-340) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 33.7 kDa Purity: >85%)
Testing Data
(HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
NCBI and Uniprot Product Information
NCBI Description
Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.
Protein type: Nucleolus; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; PLK family
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: centrosome; Golgi stack; cell soma; cytoplasm; dendrite; nucleolus; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; ATP binding
Biological Process: apoptosis; mitotic cell cycle checkpoint; negative regulation of transcription from RNA polymerase II promoter; endomitotic cell cycle; response to osmotic stress; protein amino acid phosphorylation; regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; response to reactive oxygen species; response to radiation; protein kinase B signaling cascade; G2/M transition of mitotic cell cycle; regulation of cell division; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Research Articles on PLK3
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Product Notes
The PLK3 plk3 (Catalog #AAA389416) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PLK3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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