Phospholipase B Recombinant Protein | PLB recombinant protein

Recombinant Phospholipase B (PLB)

Cat. Num. AAA2030040

• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Phospholipase B; Recombinant Phospholipase B (PLB); PLB recombinant protein

• Host: E.coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: > 95%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 250ug/mL (varies by lot)
• Sequence: MPITC PTQNEPFLRTPRNSNYTYPI KPAIENWGSD FLCTEWKASN SVPTSVHQLR PADIKVVAALGDSLTTAVGA RPNNSSDLPT SWRGLSWSIG GDGNLETHTT LPNILKKFNPYLLGFSTSTW EGTAGLNVAA EGARARDMPA QAWDLVERMK NSPDINLEKDWKLVTLFIGV NDLCHYCENP EAHLATEYVQ HIQQALDILS EELPRAFVNVVEVMELASLY QGQGGKCAML AAQNNCTCLR HSQSSLEKQE LKKVNWNLQHGISSFSYWHQ YTQREDFAVV VQPFFQNTLT PLNERGDTDL TFFSEDCFHFSDRGHAEMAI ALWNNMLEPV GRKTTSNNFT HSRAKLKCPS PESPYLYTLRNSRLLPDQAE EAPEVLYWAV PVAAGVGLVV GIIGTVVWRC RRGGRREDPPMSLRTVAL
• Sequence Length: 1463

• Applicable Applications for PLB recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Met1036~Leu1458
• Tags: N-terminal His Tag
• Subcellular Location: Membrane
• Traits: Freeze-dried powder
• Predicted isoelectric point: 5.9
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Gene Sequence

NCBI and Uniprot Product Information

• NCBI GI #: 3172337
• Molecular Weight: Predicted Molecular Mass: 51.0kDa; Accurate Molecular Mass: 51kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: phospholipase B
• UniProt Protein Name: Phospholipase B1, membrane-associated
• Protein Family: Phospholipase
• UniProt Gene Name: PLB1
• UniProt Synonym Gene Names: PLB; Phospholipase B; PLB/LIP
• UniProt Entry Name: PLB1_CAVPO

Uniprot Description

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids ().