Typical Testing Data/Standard Curve (for reference only)
Human Nesfatin 1/Nucleobindin 2 ELISA Kit | NES1/NUCB2 elisa kit
Human Nesfatin 1/Nucleobindin 2 ELISA Kit
Gene Names
NUCB2; NEFA; HEL-S-109
Reactivity
Human
Synonyms
Nesfatin 1/Nucleobindin 2; Human Nesfatin 1/Nucleobindin 2 ELISA Kit; NES1/NUCB2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NUCB2. No significant cross-reactivity or interference between NUCB2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NUCB2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NES1/NUCB2 elisa kit
Intended Uses: This NUCB2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NUCB2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: NUCB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NUCB2 antibody and an NUCB2-HRP conjugate. The assay sample and buffer are incubated together with NUCB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUCB2 concentration since NUCB2 from samples and NUCB2-HRP conjugate compete for the anti-NUCB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUCB2 from the sample, fewer sites are left to bind NUCB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUCB2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: NUCB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NUCB2 antibody and an NUCB2-HRP conjugate. The assay sample and buffer are incubated together with NUCB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUCB2 concentration since NUCB2 from samples and NUCB2-HRP conjugate compete for the anti-NUCB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUCB2 from the sample, fewer sites are left to bind NUCB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUCB2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,201 Da
NCBI Official Full Name
nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
NCBI Protein Information
nucleobindin-2; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109
UniProt Protein Name
Nucleobindin-2
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA
UniProt Entry Name
NUCB2_HUMAN
NCBI Description
This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]
Uniprot Description
NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Vesicle
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nuclear envelope; cytosol
Molecular Function: protein binding; DNA binding; calcium ion binding
Research Articles on NES1/NUCB2
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Product Notes
The Human NES1/NUCB2 nucb2 (Catalog #AAA746526) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746526 ELISA Kit recognizes Human NES1/NUCB2. It is sometimes possible for the material contained within the vial of "Nesfatin 1/Nucleobindin 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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