Rat Phospholipase B ELISA Kit | PLB elisa kit

Rat Phospholipase B ELISA Kit

Cat. Num. AAA748118

• Reactivity: Rat
• Synonyms: Phospholipase B; Rat Phospholipase B ELISA Kit; PLB elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This PLB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PLB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLB elisa kit

Principle of the assay: PLB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLB antibody and an PLB-HRP conjugate. The assay sample and buffer are incubated together with PLB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLB concentration since PLB from samples and PLB-HRP conjugate compete for the anti-PLB antibody binding site. Since the number of sites is limited, as more sites are occupied by PLB from the sample, fewer sites are left to bind PLB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PLB elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 3172337
• Molecular Weight: 162,176 Da
• NCBI Official Full Name: phospholipase B
• UniProt Protein Name: Phospholipase B1, membrane-associated
• Protein Family: Phospholipase
• UniProt Gene Name: PLB1
• UniProt Synonym Gene Names: PLB; Phospholipase B; PLB/LIP
• UniProt Entry Name: PLB1_CAVPO

Uniprot Description

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids ().