PLAUR / CD87 / uPAR recombinant protein

Recombinant Mouse PLAUR / CD87 / uPAR Protein (His & Fc tag)

Cat. Num. AAA2547135

• Gene Names: Plaur; Cd87; uPAR; u-PAR
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: PLAUR / CD87 / uPAR; Recombinant Mouse PLAUR / CD87 / uPAR Protein (His & Fc tag); Plaur; Cd87; u-PAR; uPAR; PLAUR / CD87 / uPAR recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 327

• Application Notes: The recombinant mouse PLAUR/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 522 amino acids and has a predicted molecular mass of 58 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmPLAUR/Fc monomer is approximately 80-90 kDa due to glycosylation.
• Predicted N Terminal: Leu 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for PLAUR / CD87 / uPAR recombinant protein

Background: Urokinase plasminogen activator (uPA) and/or its receptor (uPAR) are essential for metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumours. uPAR and uPA levels in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several type s of human cancer. This system has classically been thought to drive tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells, and intervening with this proteolysis by targeting uPAR has been proposed to represent a novel approach for inhibiting tumor progression. uPAR, also known as PLAUR or CD87, has been implicated in the growth, metastasis, and angiogenesis of several solid and hemotologic malignancies. uPAR is a highly glycosylated, 55-60kDa integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It is part of a cell surface system that also consists of the serine protease uPA and several specific inhibitors (plasminogen activator inhibitors 1 and 2). Additionally, the analysis of CD87 (urokinase-type plasminogen activator receptor - uPAR) expression has a potential role in the prognostic work-up of several hematological malignancies, particularly acute leukemia and multiple myeloma.

Description: A DNA sequence encoding the extracellular domain (Met 1-Thr 297) of mouse PLAUR (NP_035243.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7242179
• NCBI GeneID: 18793
• NCBI Accession #: NP_035243.1
• NCBI GenBank Nucleotide #: NM_011113.4
• UniProt Accession #: P35456; P35457
• Molecular Weight: 24,619 Da
• NCBI Official Full Name: urokinase plasminogen activator surface receptor
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: Plaur
• NCBI Official Synonym Symbols: Cd87; uPAR; u-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: Plaur
• UniProt Synonym Gene Names: U-PAR; uPAR

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 7|7 A3

Cellular Component: focal adhesion; integral to plasma membrane; plasma membrane

Molecular Function: enzyme binding; protein binding; protein domain specific binding; receptor binding; U-plasminogen activator receptor activity

Biological Process: mesenchymal cell differentiation; negative regulation of apoptosis; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of protein amino acid phosphorylation