uPAR/PLAUR Active Protein | PLAUR active protein

uPAR/PLAUR Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122572

• Gene Names: Plaur; Cd87; uPAR; u-PAR
• Purity: >97% as determined by SDS-PAGE
• Synonyms: uPAR/PLAUR; uPAR/PLAUR Protein; Mouse; Recombinant (His Tag); Mouse PLAUR/CD87/uPAR Protein (His Tag); Cd87 Protein; u-PAR Protein; uPAR Protein; plasminogen activator; urokinase receptor; PLAUR active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr297

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse PLAUR (NP_035243.1) precursor (Met 1-Thr 297) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to bind with uPA-His in a functional ELISA.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to bind with uPA-His in a functional ELISA.)

Related Product Information for PLAUR active protein

Background: Urokinase plasminogen activator (uPA) and/or its receptor (uPAR) are essential for metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumours. uPAR and uPA levels in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several types of human cancer. This system has classically been thought to drive tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells, and intervening with this proteolysis by targeting uPAR has been proposed to represent a novel approach for inhibiting tumor progression. uPAR, also known as PLAUR or CD87, has been implicated in the growth, metastasis, and angiogenesis of several solid and hemotologic malignancies. uPAR is a highly glycosylated, 55-60kDa integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It is part of a cell surface system that also consists of the serine protease uPA and several specific inhibitors (plasminogen activator inhibitors 1 and 2). Additionally, the analysis of CD87 (urokinase-type plasminogen activator receptor - uPAR) expression has a potential role in the test or prognostic work-up of several hematological malignancies, particularly acute leukemia and multiple myeloma.

Product Categories/Family for PLAUR active protein

Serine Proteases and Regulators

References

Romer J, et al. (2004) The urokinase receptor as a potential target in cancer therapy. Curr Pharm Des. 10(19): 2359-76.Bn MC, et al. (2004) CD87 (urokinase-type plasminogen activator receptor), function and pathology in hematological disorders: a review. Leukemia. 18(3): 394-400.Pillay V, et al. (2007) The urokinase plasminogen activator receptor as a gene therapy target for cancer. Trends Biotechnol. 25(1): 33-9.Mazar AP. (2008) Urokinase plasminogen activator receptor choreographs multiple ligand interactions: implications for tumor progression and therapy. Clin Cancer Res. 14(18): 5649-55.

NCBI and Uniprot Product Information

• NCBI GI #: 7242179
• NCBI GeneID: 18793
• NCBI Accession #: NP_035243.1
• NCBI GenBank Nucleotide #: NM_011113.3
• UniProt Accession #: P35456; P35457
• Molecular Weight: 160,000
• NCBI Official Full Name: urokinase plasminogen activator surface receptor
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: Plaur
• NCBI Official Synonym Symbols: Cd87; uPAR; u-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor; urokinase plasminogen activator receptor; urokinase-type plasminogen activator receptor
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• Protein Family: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: Plaur
• UniProt Synonym Gene Names: U-PAR; uPAR
• UniProt Entry Name: UPAR_MOUSE

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, GPI anchor

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein domain specific binding; protein binding; U-plasminogen activator receptor activity; enzyme binding; receptor binding

Biological Process: mesenchymal cell differentiation; negative regulation of apoptosis