Tissue Plasminogen Activator Recombinant Protein | PLAT recombinant protein

Tissue Plasminogen Activator, Recombinant, Human (tPA)

Cat. Num. AAA637506

• Gene Names: PLAT; TPA; T-PA
• Purity: Highly Purified; 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Tissue Plasminogen Activator; Recombinant; Human (tPA); PLAT recombinant protein

• Host: CHO cells
• Purity/Purification: Highly Purified; 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder in L-arginine, phosphoric acid, Tween 80. Reconstitute with sterile ddH2O, 0.1% HSA or BSA, to 100ug/ml.
• Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ser-Tyr-Gln-Val-Ile-Cys.

• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile dH2O, 0.1% HSA or BSA. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PLAT recombinant protein

Recombinant Human Tissue Plasminogen Activator produced in Chinese Hamster Ovary Cells (CHO) is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. Human TPA is a serine protease enzyme that converts plasminogen to plasmin.

Product Categories/Family for PLAT recombinant protein

Molecular Biology; MB-Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 28274638
• NCBI GeneID: 5327
• UniProt Accession #: P00750; Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
• Molecular Weight: 59kD
• NCBI Official Full Name: tissue plasminogen activator
• NCBI Official Synonym Full Names: plasminogen activator, tissue
• NCBI Official Symbol: PLAT
• NCBI Official Synonym Symbols: TPA; T-PA
• NCBI Protein Information: tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
• UniProt Protein Name: Tissue-type plasminogen activator
• Protein Family: Tissue-type plasminogen activator
• UniProt Gene Name: PLAT
• UniProt Synonym Gene Names: t-PA; t-plasminogen activator; tPA
• UniProt Entry Name: TPA_HUMAN

NCBI Description

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Vesicle; EC 3.4.21.68; Cytoskeletal; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: negative regulation of proteolysis; response to peptide hormone stimulus; synaptic transmission, glutamatergic; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; protein modification process; regulation of synaptic plasticity; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator