Typical Testing Data/Standard Curve (for reference only)
Human Placental Alkaline Phosphatase ELISA Kit | PLAP elisa kit
Human Placental Alkaline Phosphatase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PLAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PLAP antibody and an PLAP-HRP conjugate. The assay sample and buffer are incubated together with PLAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLAP concentration since PLAP from samples and PLAP-HRP conjugate compete for the anti-PLAP antibody binding site. Since the number of sites is limited, as more sites are occupied by PLAP from the sample, fewer sites are left to bind PLAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLAP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]
Protein type: EC 3.1.3.1; Cell surface; Motility/polarity/chemotaxis; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Membrane protein, GPI anchor; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 2q37
Cellular Component: cell surface; plasma membrane; integral to membrane
Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding
Biological Process: dephosphorylation
Research Articles on PLAP
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Product Notes
The Human PLAP alpp (Catalog #AAA728565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728565 ELISA Kit recognizes Human PLAP. It is sometimes possible for the material contained within the vial of "Placental Alkaline Phosphatase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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