Typical Testing Data/Standard Curve (for reference only)
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Mouse Sodium/Glucose Cotransporter 1 ELISA Kit | SGLT1 elisa kit
Mouse Sodium/Glucose Cotransporter 1 ELISA Kit
Gene Names
Slc5a1; Sglt1
Reactivity
Mouse
Synonyms
Sodium/Glucose Cotransporter 1; Mouse Sodium/Glucose Cotransporter 1 ELISA Kit; SGLT1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of SGLT1. No significant cross-reactivity or interference between SGLT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SGLT1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for SGLT1 elisa kit
Intended Uses: This SGLT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SGLT1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SGLT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SGLT1 antibody and an SGLT1-HRP conjugate. The assay sample and buffer are incubated together with SGLT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SGLT1 concentration since SGLT1 from samples and SGLT1-HRP conjugate compete for the anti-SGLT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SGLT1 from the sample, fewer sites are left to bind SGLT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SGLT1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SGLT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SGLT1 antibody and an SGLT1-HRP conjugate. The assay sample and buffer are incubated together with SGLT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SGLT1 concentration since SGLT1 from samples and SGLT1-HRP conjugate compete for the anti-SGLT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SGLT1 from the sample, fewer sites are left to bind SGLT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SGLT1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,449 Da
NCBI Official Full Name
sodium/glucose cotransporter 1
NCBI Official Synonym Full Names
solute carrier family 5 (sodium/glucose cotransporter), member 1
NCBI Official Symbol
Slc5a1
NCBI Official Synonym Symbols
Sglt1
NCBI Protein Information
sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; sodium glucose cotransporter 1; sodium-glucose cotransporter 1; solute carrier family 5 member 1; solute carrier family 5, member 1; solute carrier family 5, member alpha 1; high affinity sodium-glucose cotransporter
UniProt Protein Name
Sodium/glucose cotransporter 1
Protein Family
UniProt Gene Name
Slc5a1
UniProt Synonym Gene Names
Na(+)/glucose cotransporter 1
UniProt Entry Name
SC5A1_MOUSE
Uniprot Description
SLC5A1: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. Expressed mainly in intestine and kidney. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family
Cellular Component: membrane; brush border membrane; apical plasma membrane; integral to membrane; intercellular junction; brush border
Molecular Function: glucose:sodium symporter activity; transporter activity; symporter activity; glucose transmembrane transporter activity
Biological Process: transport; carbohydrate transport; sodium ion transport; ion transport; glucose transport; metanephros development; transmembrane transport; intestinal absorption
Research Articles on SGLT1
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Product Notes
The Mouse SGLT1 slc5a1 (Catalog #AAA750205) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA750205 ELISA Kit recognizes Mouse SGLT1. It is sometimes possible for the material contained within the vial of "Sodium/Glucose Cotransporter 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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