Typical Testing Data/Standard Curve (for reference only)
Human Protease Activated Receptor 1 ELISA Kit | PAR1 elisa kit
Human Protease Activated Receptor 1 ELISA Kit
Reactivity
Human
Synonyms
Protease Activated Receptor 1; Human Protease Activated Receptor 1 ELISA Kit; PAR1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PAR1. No significant cross-reactivity or interference between PAR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PAR1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PAR1 elisa kit
Intended Uses: This PAR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PAR1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: PAR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PAR1 antibody and an PAR1-HRP conjugate. The assay sample and buffer are incubated together with PAR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAR1 concentration since PAR1 from samples and PAR1-HRP conjugate compete for the anti-PAR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAR1 from the sample, fewer sites are left to bind PAR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAR1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PAR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PAR1 antibody and an PAR1-HRP conjugate. The assay sample and buffer are incubated together with PAR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAR1 concentration since PAR1 from samples and PAR1-HRP conjugate compete for the anti-PAR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAR1 from the sample, fewer sites are left to bind PAR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAR1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PAR1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
41,712 Da
NCBI Official Full Name
sex hormone-binding globulin
UniProt Protein Name
Sex hormone-binding globulin
Protein Family
UniProt Gene Name
SHBG
UniProt Synonym Gene Names
SHBG; SBP; ABP; TeBG
UniProt Entry Name
SHBG_HUMAN
Uniprot Description
SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: extracellular region
Molecular Function: androgen binding
Biological Process: primary spermatocyte growth
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Product Notes
The Human PAR1 shbg (Catalog #AAA733919) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA733919 ELISA Kit recognizes Human PAR1. It is sometimes possible for the material contained within the vial of "Protease Activated Receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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