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Typical Testing Data/Standard Curve (for reference only)

Rat Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma ELISA Kit | Pip4k2c elisa kit

Rat Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma ELISA Kit

Gene Names
Pip4k2c; Pip5k2c
Reactivity
Rat
Synonyms
Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma; Rat Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma ELISA Kit; Pip4k2c elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay recognizes recombinant and natural rat Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma. No significant cross-reactivity or interference was observed.
Sequence Length
421
Assay Type
Sandwich
Detection Range
0.156-10 ng/ml
Sensitivity
0.085 ng/mL
Intra-Assay CV
<=6.7%
Inter-Assay CV
<=7.8%
Recovery
89%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Pip4k2c elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,049 Da
NCBI Official Full Name
phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
NCBI Official Synonym Full Names
phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
NCBI Official Symbol
Pip4k2c
NCBI Official Synonym Symbols
Pip5k2c
NCBI Protein Information
phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
UniProt Protein Name
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
UniProt Gene Name
Pip4k2c
UniProt Synonym Gene Names
Pip5k2c; PI(5)P 4-kinase type II gamma; PIP4KII-gamma
UniProt Entry Name
PI42C_RAT

NCBI Description

may play a role in phosphatidylinositol bisphosphate synthesis in the endoplasmic reticulum; has similarity to phosphatidylinositol 5-phosphate 4-kinase IIalpha and IIbeta isoforms [RGD, Feb 2006]

Uniprot Description

PIP4K2C: May play an important role in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.149; Endoplasmic reticulum; Kinase, lipid; Motility/polarity/chemotaxis

Cellular Component: autophagic vacuole; endoplasmic reticulum

Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; identical protein binding

Biological Process: regulation of autophagy

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Product Notes

The Rat Pip4k2c pip4k2c (Catalog #AAA288275) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288275 ELISA Kit recognizes Rat Pip4k2c. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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