Mouse Kelch repeat and BTB domain-containing protein 13 ELISA Kit | Kbtbd13 elisa kit

Mouse Kelch repeat and BTB domain-containing protein 13 ELISA Kit

Cat. Num. AAA288481

• Gene Names: Kbtbd13; 5430433E21Rik
• Reactivity: Mouse
• Synonyms: Kelch repeat and BTB domain-containing protein 13; Mouse Kelch repeat and BTB domain-containing protein 13 ELISA Kit; Kbtbd13 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Kelch repeat and BTB domain-containing protein 13. No significant cross-reactivity or interference was observed.
• Sequence Length: 458

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.085 ng/mL
• Intra-Assay CV: <=4.2%
• Inter-Assay CV: <=7.1%
• Recovery: 89%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Kbtbd13 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch repeat and BTB domain-containing protein 13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Kelch repeat and BTB domain-containing protein 13 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kelch repeat and BTB domain-containing protein 13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Kelch repeat and BTB domain-containing protein 13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157384988
• NCBI GeneID: 74492
• NCBI Accession #: NP_083250.1
• NCBI GenBank Nucleotide #: NM_028974.1
• UniProt Accession #: Q8C828
• Molecular Weight: 49,769 Da
• NCBI Official Full Name: kelch repeat and BTB domain-containing protein 13
• NCBI Official Synonym Full Names: kelch repeat and BTB (POZ) domain containing 13
• NCBI Official Symbol: Kbtbd13
• NCBI Official Synonym Symbols: 5430433E21Rik
• NCBI Protein Information: kelch repeat and BTB domain-containing protein 13
• UniProt Protein Name: Kelch repeat and BTB domain-containing protein 13
• Protein Family: Kelch repeat and BTB domain-containing protein
• UniProt Gene Name: Kbtbd13
• UniProt Entry Name: KBTBD_MOUSE

Uniprot Description

KBTBD13: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. Defects in KBTBD13 are the cause of nemaline myopathy type 6 (NEM6). A form of nemaline myopathy characterized by childhood onset of slowly progressive proximal muscle weakness, exercise intolerance, and slow movements with stiff muscles. Patients are unable to run or correct themselves from falling over.

Cellular Component: cytoplasm

Molecular Function: ubiquitin-protein ligase activity