Paired Immunoglobulin-Like Type 2 Receptor alpha Recombinant Protein | Pilra recombinant protein
Paired Immunoglobulin-Like Type 2 Receptor alpha, Recombinant, Mouse (PILR-alpha, PILRa)
Gene Names
Pilra; FDF03; AV021745
Reactivity
Mouse
Applications
Western Blot
Purity
Highly Purified95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
Synonyms
Paired Immunoglobulin-Like Type 2 Receptor alpha; Recombinant; Mouse (PILR-alpha; PILRa); Pilra recombinant protein
Reactivity
Mouse
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 0.1mg/ml.
Applicable Applications for Pilra recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 20.5kD migrates as an ~ 40-50kD protein in SDS-PAGE under reducing conditions.
Dilution: Western Blot: 20.5kD migrates as an ~ 40-50kD protein in SDS-PAGE under reducing conditions.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Pilra recombinant protein
PILR-alpha (paired immunoglobulin-like type 2 receptor-alpha; also FDF03) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-Beta and it likely gave rise to PILR-Beta through gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-alpha and -Beta should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-alpha and -Beta show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-alpha is a monomeric, 271 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a long, 83 aa cytoplasmic region. The ECD shows one V-type Ig-like domain between aa 39-157, while the cytoplasmic region contains two ITIMs (immunoreceptor Tyr-based inhibitory motifs) between aa's 265-270 and 294-299. Given that ITIMs are known to interact with phosphatases such as PTPN6 and PTPN11, the presence of these motifs makes mouse PILR-alpha an inhibitory receptor. In human, activation of PILR-alpha inhibits CD32/FcgammaRII-induced calcium mobilization (3). Although CD99 is a known ligand for both PILR-alpha and -Beta (4), highest affinity binding seems to occur between CD99 and PILR-alpha (4). Mouse PILR-alpha is found on neutrophils and macrophages (4). Mouse PILR-alpha ECD is 43% and 69% aa identical to human and rat PILR-alpha ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-Beta (3). One Potential isoform of PILR-alpha has been reported. It varies only within the first 28 aa of the signal sequence (5).
Product Categories/Family for Pilra recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20.5kD
NCBI Official Full Name
paired immunoglobulin-like type 2 receptor alpha
NCBI Official Synonym Full Names
paired immunoglobin-like type 2 receptor alpha
NCBI Official Symbol
Pilra
NCBI Official Synonym Symbols
FDF03; AV021745
NCBI Protein Information
paired immunoglobulin-like type 2 receptor alpha; cell surface receptor FDF03; inhibitory receptor PILR-alpha
UniProt Protein Name
Paired immunoglobulin-like type 2 receptor alpha
Protein Family
UniProt Gene Name
Pilra
UniProt Entry Name
PILRA_MOUSE
Uniprot Description
PILRA: Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILRA is thought to act as a cellular signaling inhibitory receptor by recruiting cytoplasmic phosphatases like PTPN6/SHP-1 and PTPN11/SHP-2 via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Receptor for PIANP. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding
Biological Process: signal transduction
Research Articles on Pilra
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Product Notes
The Pilra pilra (Catalog #AAA637255) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The Paired Immunoglobulin-Like Type 2 Receptor alpha, Recombinant, Mouse (PILR-alpha, PILRa) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Paired Immunoglobulin-Like Type 2 Receptor alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 20.5kD migrates as an ~ 40-50kD protein in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the Pilra pilra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paired Immunoglobulin-Like Type 2 Receptor alpha, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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