Mouse SOCS3 (Suppressors Of Cytokine Signaling 3) CLIA Kit | SOCS3 clia kit

Mouse SOCS3 (Suppressors Of Cytokine Signaling 3) CLIA Kit

Cat. Num. AAA2532974

• Gene Names: SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• Reactivity: Mouse
• Synonyms: SOCS3 (Suppressors Of Cytokine Signaling 3); Mouse SOCS3 (Suppressors Of Cytokine Signaling 3) CLIA Kit; SOCS3 clia kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant SOCS3. No significant cross-reactivity or interference between SOCS3 and analogues was observed.
• Sequence Length: 225

• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOCS3 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of SOCS3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to SOCS3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SOCS3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SOCS3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of SOCS3. You can calculate the concentration of SOCS3 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45439352
• NCBI GeneID: 9021
• NCBI Accession #: NP_003946.3
• NCBI GenBank Nucleotide #: NM_003955.4
• Molecular Weight: 24,770 Da
• NCBI Official Full Name: suppressor of cytokine signaling 3
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 3
• NCBI Official Symbol: SOCS3
• NCBI Official Synonym Symbols: CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• NCBI Protein Information: suppressor of cytokine signaling 3
• UniProt Protein Name: Suppressor of cytokine signaling 3
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS3
• UniProt Synonym Gene Names: CIS3; SSI3; SOCS-3; CIS-3; SSI-3
• UniProt Entry Name: SOCS3_HUMAN

NCBI Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]

Uniprot Description

SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; protein kinase inhibitor activity

Biological Process: response to food; response to drug; organ regeneration; cytokine and chemokine mediated signaling pathway; response to glucocorticoid stimulus; protein ubiquitination; negative regulation of insulin receptor signaling pathway; response to lipopolysaccharide; JAK-STAT cascade; response to estradiol stimulus; response to insulin stimulus; response to heat; negative regulation of inflammatory response; regulation of growth; response to hypoxia; response to gamma radiation; positive regulation of cell differentiation; response to progesterone stimulus; negative regulation of JAK-STAT cascade; negative regulation of apoptosis; aging

Disease: Dermatitis, Atopic, 4