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Human GPI ethanolamine phosphate transferase 3 ELISA Kit | PIGO elisa kit

Human GPI ethanolamine phosphate transferase 3 (PIGO) ELISA Kit

Gene Names
PIGO; HPMRS2
Reactivity
Human
Synonyms
GPI ethanolamine phosphate transferase 3; Human GPI ethanolamine phosphate transferase 3 (PIGO) ELISA Kit; PIGO elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PIGO. No significant cross-reactivity or interference between Human PIGO and analogues was observed.
Sequence Length
1089
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PIGO elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIGO in samples. An antibody specific for PIGO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIGO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGO bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,992 Da
NCBI Official Full Name
GPI ethanolamine phosphate transferase 3 isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis class O
NCBI Official Symbol
PIGO
NCBI Official Synonym Symbols
HPMRS2
NCBI Protein Information
GPI ethanolamine phosphate transferase 3
UniProt Protein Name
GPI ethanolamine phosphate transferase 3
UniProt Gene Name
PIGO
UniProt Synonym Gene Names
PIG-O

NCBI Description

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PIGO: Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI third mannose which links the GPI-anchor to the C-terminus of the proteins by an amide bond. Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.-.-.-; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: endoplasmic reticulum membrane; integral component of membrane; membrane

Molecular Function: mannose-ethanolamine phosphotransferase activity

Biological Process: GPI anchor biosynthetic process

Disease: Hyperphosphatasia With Mental Retardation Syndrome 2

Research Articles on PIGO

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Product Notes

The Human PIGO pigo (Catalog #AAA281646) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281646 ELISA Kit recognizes Human PIGO. It is sometimes possible for the material contained within the vial of "GPI ethanolamine phosphate transferase 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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