PDGF-BB active protein

PDGF-BB Protein (Active)

Cat. Num. AAA355607

• Gene Names: PDGFB; SIS; SSV; PDGF2; c-sis; PDGF-2
• Applications: SDS-Page
• Synonyms: PDGF-BB; PDGF-BB Protein (Active); PDGF-BB active protein

• Host: Pichia yeast
• Form/Format: Lyophilized

• Applicable Applications for PDGF-BB active protein: HPLC, SDS-PAGE
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is < 1 ng/ml corresponding to a specific activity of 1 × 106 IU/mg.
• Dimers: < 1% by SDS-PAGE
• Endotoxin Level: < 0.1 ng/ug (1 EU/ug) by LAL test
• Reconstitution: Reconstitute with sterile 18MΩ-cm H2O.
• Storage Buffer: Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.
• Preparation and Storage: Ship at 4 degree C. Upon receipt, store at -20 degree C or -80 degree C for 2 years. After reconstituting, it can store at 4 degree C for a week, it is recommended to add 0.1% HSA or BSA for long term. Avoid repeated freeze and thaw cycles

Related Product Information for PDGF-BB active protein

Recombinant human PDGF-BB protein with molecular weight 28 kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 15451786
• NCBI GeneID: 5155
• NCBI Accession #: NP_148937.1
• NCBI GenBank Nucleotide #: NM_033016.2
• UniProt Accession #: P01127; P78431; Q15354; Q6FHE7; Q9UF23; G3XAG8
• Molecular Weight: 27,283 Da
• NCBI Official Full Name: platelet-derived growth factor subunit B isoform 2 preproprotein
• NCBI Official Synonym Full Names: platelet-derived growth factor beta polypeptide
• NCBI Official Symbol: PDGFB
• NCBI Official Synonym Symbols: SIS; SSV; PDGF2; c-sis; PDGF-2
• NCBI Protein Information: platelet-derived growth factor subunit B; becaplermin; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• UniProt Protein Name: Platelet-derived growth factor subunit B
• UniProt Gene Name: PDGFB
• UniProt Synonym Gene Names: PDGF2; SIS; PDGF subunit B
• UniProt Entry Name: PDGFB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA

By similarity.

Subunit structure: Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1

By similarity. Ref.19 Ref.24

Subcellular location: Secreted. Note: Released by platelets upon wounding.

Tissue specificity: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. Ref.20

Involvement in disease: A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Ref.21

Pharmaceutical use: Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.