Rabbit anti-Human FPRL2 Polyclonal Antibody | anti-FPRL2 antibody

FPRL2 (Formyl Peptide Receptor-like 2, FMLP-related Receptor II, FMLP-R-II, N-formyl Peptide Receptor 3, FPR3, FPRH1) (Azide free) (HRP)

Cat. Num. AAA6476768

• Gene Names: FPR3; FMLPY; FPRH1; FPRH2; FPRL2; RMLP-R-I; FML2_HUMAN
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FPRL2; Polyclonal Antibody; FPRL2 (Formyl Peptide Receptor-like 2; FMLP-related Receptor II; FMLP-R-II; N-formyl Peptide Receptor 3; FPR3; FPRH1) (Azide free) (HRP); anti-FPRL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FPRL2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FPRL2 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: IHC-P: 1:50-1:100; ELISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from between a portion within aa313-342 in the center region of human FPRL2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FPRL2 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). FPRL2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with FPRL2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FPRL2 Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FPRL2 antibody

Antibodies; Receptors

References

1. Yang, D., et al., J. Leukoc. Biol. 72(3): 598-607 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 38455413
• NCBI GeneID: 2359
• NCBI Accession #: NP_002021.3
• NCBI GenBank Nucleotide #: NM_002030.3
• UniProt Accession #: P25089
• Molecular Weight: 39,965 Da
• NCBI Official Full Name: N-formyl peptide receptor 3
• NCBI Official Synonym Full Names: formyl peptide receptor 3
• NCBI Official Symbol: FPR3
• NCBI Official Synonym Symbols: FMLPY; FPRH1; FPRH2; FPRL2; RMLP-R-I; FML2_HUMAN
• NCBI Protein Information: N-formyl peptide receptor 3; FMLP-R-II; FMLP-related receptor II; formyl peptide receptor-like 2
• UniProt Protein Name: N-formyl peptide receptor 3
• UniProt Gene Name: FPR3
• UniProt Synonym Gene Names: FPRH1; FPRL2; FMLP-R-II
• UniProt Entry Name: FPR3_HUMAN

Uniprot Description

FPR3: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: integral to membrane; plasma membrane

Molecular Function: N-formyl peptide receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell motility; chemotaxis; signal transduction