Human Phosphorylated adapter RNA export protein ELISA Kit | PHAX elisa kit

Human Phosphorylated adapter RNA export protein (PHAX) ELISA Kit

Cat. Num. AAA280305

• Gene Names: PHAX; RNUXA
• Reactivity: Human
• Synonyms: Phosphorylated adapter RNA export protein; Human Phosphorylated adapter RNA export protein (PHAX) ELISA Kit; PHAX elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PHAX. No significant cross-reactivity or interference between Human PHAX and analogues was observed.
• Sequence Length: 394

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PHAX elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PHAX in samples. An antibody specific for PHAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHAX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHAX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHAX bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 66392146
• NCBI GeneID: 51808
• NCBI Accession #: NP_115553.2
• NCBI GenBank Nucleotide #: NM_032177.3
• UniProt Accession #: Q9H814; Q9H8W1
• Molecular Weight: 44,403 Da
• NCBI Official Full Name: phosphorylated adapter RNA export protein
• NCBI Official Synonym Full Names: phosphorylated adaptor for RNA export
• NCBI Official Symbol: PHAX
• NCBI Official Synonym Symbols: RNUXA
• NCBI Protein Information: phosphorylated adapter RNA export protein
• UniProt Protein Name: Phosphorylated adapter RNA export protein
• Protein Family: Phosphorylated adapter RNA export protein
• UniProt Gene Name: PHAX
• UniProt Synonym Gene Names: RNUXA

Uniprot Description

RNUXA: A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation- independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA. Belongs to the PHAX family.

Protein type: Nuclear import; RNA-binding

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: Cajal body; cell soma; centrosome; cytosol; nucleoplasm; nucleus

Molecular Function: protein binding; RNA binding; toxin binding

Biological Process: nuclear export; protein transport; snRNA export from nucleus; snRNA transcription from RNA polymerase II promoter