Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (PPP3CB) Standard Curve.)
Human Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit beta Isoform (PPP3CB) ELISA Kit | PPP3CB elisa kit
Human Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit beta Isoform (PPP3CB) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (PPP3CB) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (PPP3CB) Standard Curve.)
Background: Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca(2+)-binding regulatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B (PPP3R1) in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha (PPP3CA) and beta (PPP3CB), of calcineurin A encoded by separate genes located on different human chromosomes. A third isoform, A-gamma (PPP3CC), is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs.
NCBI and Uniprot Product Information
Uniprot Description
PPP3CB: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A beta. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively.
Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 10q22.2
Cellular Component: nucleoplasm; T-tubule; plasma membrane; Z disc; cytosol; calcineurin complex
Molecular Function: protein dimerization activity; calmodulin binding; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; calcium ion binding; protein serine/threonine phosphatase activity; drug binding; protein phosphatase 2B binding
Biological Process: T cell activation; positive regulation of NFAT protein import into nucleus; axon extension; heart development; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; learning; social behavior; signal transduction; protein amino acid dephosphorylation; protein amino acid phosphorylation; memory; calcium ion-dependent exocytosis; locomotion during locomotory behavior; T cell proliferation; calcineurin-NFAT signaling pathway; dephosphorylation; response to cytokine stimulus; negative regulation of T cell mediated cytotoxicity; innate immune response; T cell homeostasis; positive regulation of transcription from RNA polymerase II promoter; regulation of insulin secretion; T cell differentiation
Research Articles on PPP3CB
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Product Notes
The Human PPP3CB ppp3cb (Catalog #AAA9714855) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714855 ELISA Kit recognizes Human PPP3CB. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit beta Isoform (PPP3CB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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