Phosphoglycerate Mutase 1 Recombinant Protein | PGAM1 recombinant protein

Recombinant Phosphoglycerate Mutase 1, Brain (PGAM1)

Cat. Num. AAA2031672

• Gene Names: PGAM1; PGAMA; PGAM-B; HEL-S-35
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Phosphoglycerate Mutase 1; Recombinant Phosphoglycerate Mutase 1; Brain (PGAM1); PGAM1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; AAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVRKAMEAVAAQG KAKK
• Sequence Length: 254

• Applicable Applications for PGAM1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.4kDa
• Accurate Molecular Mass (KD): 32kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ala2~Lys254
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4505753
• NCBI GeneID: 5223
• NCBI Accession #: NP_002620.1
• NCBI GenBank Nucleotide #: NM_002629.2
• Molecular Weight: 28,804 Da
• NCBI Official Full Name: phosphoglycerate mutase 1
• NCBI Official Synonym Full Names: phosphoglycerate mutase 1 (brain)
• NCBI Official Symbol: PGAM1
• NCBI Official Synonym Symbols: PGAMA; PGAM-B; HEL-S-35
• NCBI Protein Information: phosphoglycerate mutase 1
• UniProt Protein Name: Phosphoglycerate mutase 1
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: PGAM1
• UniProt Synonym Gene Names: PGAMA; PGAM-B
• UniProt Entry Name: PGAM1_HUMAN

NCBI Description

Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010]

Uniprot Description

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: EC 5.4.2.4; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); EC 5.4.2.11; EC 3.1.3.13

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding

Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; regulation of pentose-phosphate shunt; gluconeogenesis