Chicken Phosphofructokinase- Platelet ELISA Kit | PFKP elisa kit

Chicken Phosphofructokinase- Platelet ELISA Kit

Cat. Num. AAA737038

• Gene Names: PFKP; PFKF; PFK-C
• Reactivity: Chicken
• Synonyms: Phosphofructokinase- Platelet; Chicken Phosphofructokinase- Platelet ELISA Kit; PFKP elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PFKP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PFKP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PFKP elisa kit

Principle of the assay: PFKP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFKP antibody and an PFKP-HRP conjugate. The assay sample and buffer are incubated together with PFKP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFKP concentration since PFKP from samples and PFKP-HRP conjugate compete for the anti-PFKP antibody binding site. Since the number of sites is limited, as more sites are occupied by PFKP from the sample, fewer sites are left to bind PFKP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFKP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PFKP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 57162486
• NCBI GeneID: 5214
• UniProt Accession #: Q5VSR5; O14943; Q01813; Q49A78; Q5VSR6; Q5VSR7; Q8WTZ9
• Molecular Weight: 22,677 Da
• NCBI Official Full Name: phosphofructokinase, platelet
• NCBI Official Synonym Full Names: phosphofructokinase, platelet
• NCBI Official Symbol: PFKP
• NCBI Official Synonym Symbols: PFKF; PFK-C
• NCBI Protein Information: 6-phosphofructokinase type C; phosphohexokinase; phosphofructokinase 1; phosphofructo-1-kinase isozyme C; Phosphofructokinase, platelet type; 6-phosphofructokinase, platelet type
• UniProt Protein Name: 6-phosphofructokinase type C
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKP
• UniProt Entry Name: Q5VSR5_HUMAN

NCBI Description

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]

Uniprot Description

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; nucleus; cytosol

Molecular Function: metal ion binding; protein complex binding; 6-phosphofructokinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process