Mouse anti-Human RICTOR Monoclonal Antibody | anti-RICTOR antibody

RICTOR (Rapamycin-insensitive Companion of mTOR, AVO3 Homolog, hAVO3, KIAA1999, mAVO3, PIA) (AP)

Cat. Num. AAA6133427

• Gene Names: RICTOR; PIA; AVO3; hAVO3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RICTOR; Monoclonal Antibody; RICTOR (Rapamycin-insensitive Companion of mTOR; AVO3 Homolog; hAVO3; KIAA1999; mAVO3; PIA) (AP); DKFZp686B11164; MGC39830; anti-RICTOR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 1F3
• Specificity: Recognizes human RICTOR.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RICTOR antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Sandwich ELISA: The detection limit for recombinant GST tagged RICTOR is ~0.03ng/ml as a capture antibody.; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant protein corresponding to aa1-99 from human RICTOR (NP_689969) with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RICTOR monoclonal antibody Western Blot analysis of RICTOR expression in Hela NE.)

Western Blot (WB)

(Western Blot analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody. Lane 1: RICTOR transfected lysate (29.8kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

Immunoprecipitation (IP)

(Immunoprecipitation of RICTOR transfected lysate using RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR rabbit polyclonal antibody)

Testing Data

(Detection limit for recombinant GST tagged RICTOR is ~0.03ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot detection against Immunogen (36.89kD).)

Product Categories/Family for anti-RICTOR antibody

Antibodies; Signal Transduction

References

1. Nuclear Foxo1 Controls And Integrates Key Signaling Pathways In Hepatocytes. Naimi M, Gautier N, Chaussade C, Valverde AM, Accili D, Van Obberghen E.Endocrinology. 2007 May;148(5):2424-34. Epub 2007 Feb 15.

NCBI and Uniprot Product Information

• NCBI GI #: 59891448
• NCBI GeneID: 253260
• NCBI Accession #: NP_689969
• NCBI GenBank Nucleotide #: NM_152756
• UniProt Accession #: Q6R327; Q68DT5; Q86UB7; Q8N3A0; Q8NCM6; B2RNX0; B7ZMF7
• Molecular Weight: 195,011 Da
• NCBI Official Full Name: rapamycin-insensitive companion of mTOR isoform 1
• NCBI Official Synonym Full Names: RPTOR independent companion of MTOR, complex 2
• NCBI Official Symbol: RICTOR
• NCBI Official Synonym Symbols: PIA; AVO3; hAVO3
• NCBI Protein Information: rapamycin-insensitive companion of mTOR; AVO3 homolog; RICTOR; TORC2-specific protein AVO3; pianissimo
• UniProt Protein Name: Rapamycin-insensitive companion of mTOR
• Protein Family: Rapamycin-insensitive companion
• UniProt Gene Name: RICTOR
• UniProt Synonym Gene Names: hAVO3
• UniProt Entry Name: RICTR_HUMAN

Uniprot Description

RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.

Protein type: Cell cycle regulation; Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: TORC2 complex; cytosol

Molecular Function: protein binding; ribosome binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; actin cytoskeleton reorganization; innate immune response; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway