PF4 (CXCL4) Recombinant Protein | CXCL4 recombinant protein

Human PF4 (CXCL4) Recombinant

Cat. Num. AAA553337

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: PF4 (CXCL4); Human PF4 (CXCL4) Recombinant; Human PF4 Recombinant; PF4; CXCL4; SCYB4; oncostatin; CXCL4 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant PF4/CXCL4 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 20mM PB,1.5M NaCl pH 7.4.
• Sequence: MSSAAGFCAS RPGLLFLGLL LLPLVVAFAS AEAEEDGDLQ CLCVKTTSQV RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE S
• Sequence Length: 101

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human PF4 CXCL4 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native human PF4 is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa.; ; Recombinant PF4 is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CXCL4 recombinant protein

Platelet factor 4 (PF4/CXCL4) is an ELR− tetrameric, cationic chemokine that constitutes 25% of the protein in platelet α-granules. It is also found bound to the luminal vascular endothelial surface. Although platelets represent the primary source of PF4, it is also expressed at lower levels in other cells of the immune system including cultured T cells, monocytes, and endothelial and smooth muscle cells.

Product Categories/Family for CXCL4 recombinant protein

Cytokines

References

• cxc chemokine ligand 4 (cxcl4) down-regulates cc chemokine receptor expression on human monocytes .Innate Immunity, Nov 2010; 10.1177/1753425910388833.
• cxcl4 downregulates the atheroprotective hemoglobin receptor cd163 in human macrophages .Circ. Res., Jan 2010; 106: 203 - 211.
• platelet factor 4 (cxcl4) facilitates human macrophage infection with hiv-1 and potentiates virus replication .Innate Immunity, Dec 2009; 15: 368 - 379.
• the cathelicidin ll-37 activates human mast cells and is degraded by mast cell tryptase: counter-regulation by cxcl4 .J. Immunol., Aug 2009; 183: 2223 - 2231.
• immune complexes formed following the binding of anti–platelet factor 4 (cxcl4) antibodies to cxcl4 stimulate human neutrophil activation and cell adhesion .Blood, Aug 2008; 112: 1091 - 1100.