Human Phospho-Exiracellular Signal-Regulated Kinase ELISA Kit | PERK elisa kit

Human Phospho-Exiracellular Signal-Regulated Kinase ELISA Kit

Cat. Num. AAA14568

• Gene Names: PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
• Reactivity: Human
• Synonyms: Phospho-Exiracellular Signal-Regulated Kinase; Human Phospho-Exiracellular Signal-Regulated Kinase ELISA Kit; PERK elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human PERK and analogues was observed.
• Sequence Length: 455

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 125 pg/ml – 4000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PERK concentrations in Human serum, plasma and other body fluids. Using Purified Human PERK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PERK and PERK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PERK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PERK elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PERK concentrations in Human serum, plasma and other body fluids. Using Purified Human PERK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PERK and PERK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PERK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 193211600
• NCBI GeneID: 5494
• NCBI Accession #: NP_808821.2
• NCBI GenBank Nucleotide #: NM_177952.2
• UniProt Accession #: P35813; O75551; B5BU11; J3KNM0
• Molecular Weight: 42,448 Da
• NCBI Official Full Name: protein phosphatase 1A isoform 3
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1A
• NCBI Official Symbol: PPM1A
• NCBI Official Synonym Symbols: PP2CA; PP2Calpha; PP2C-ALPHA
• NCBI Protein Information: protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• UniProt Protein Name: Protein phosphatase 1A
• UniProt Gene Name: PPM1A
• UniProt Synonym Gene Names: PPPM1A; PP2C-alpha
• UniProt Entry Name: PPM1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; signal transducer activity; protein binding; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity

Biological Process: N-terminal protein myristoylation; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; dephosphorylation; transforming growth factor beta receptor signaling pathway; insulin receptor signaling pathway; gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; positive regulation of Wnt receptor signaling pathway