Western Blot (WB)
(Western Blot of A549 Cell Lysate using UACA / Nucling Monoclonal Antibody (AE-5))
Mouse anti-Human, Mouse UACA / Nucling Monoclonal Antibody | anti-UACA antibody
UACA / Nucling (Nuclear Membrane Marker) Mouse Monoclonal Antibody
Gene Names
UACA; NUCLING
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Synonyms
UACA / Nucling; Monoclonal Antibody; UACA / Nucling (Nuclear Membrane Marker) Mouse Monoclonal Antibody; Nuclear membrane binding protein; Uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA); NUCLING; anti-UACA antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
AE-5
Specificity
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1403
Applicable Applications for anti-UACA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1.0ug/ml)
Western Blot (0.5-1ug/ml)
Immunofluorescence (0.5-1.0ug/ml)
Western Blot (0.5-1ug/ml)
Cellular Localization
Nuclear membrane
Immunogen
Nuclei of myeloid leukemia biopsy cells
Hu-Chromosome Location
15q22-q24
Positive Control
HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Western Blot (WB)
(Western Blot of A549 Cell Lysate using UACA / Nucling Monoclonal Antibody (AE-5))
Western Blot (WB)
(Western Blot of A549 Cell Lysate using UACA / Nucling Monoclonal Antibody (AE-5))
References
Yamada, K., et al. 2001. Identification of a novel autoantigen UACA in patients with panuveitis. Biochem. Biophys. Res. Commun. 280: 1169-1176. Ohkura, T., et al. 2004. Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves disease. Biochem. Biophys. Res. Commun. 321: 432-440
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
160kDa
NCBI Official Full Name
uveal autoantigen with coiled-coil domains and ankyrin repeats isoform 2
NCBI Official Synonym Full Names
uveal autoantigen with coiled-coil domains and ankyrin repeats
NCBI Official Symbol
UACA
NCBI Official Synonym Symbols
NUCLING
NCBI Protein Information
uveal autoantigen with coiled-coil domains and ankyrin repeats
UniProt Protein Name
Uveal autoantigen with coiled-coil domains and ankyrin repeats
UniProt Gene Name
UACA
UniProt Synonym Gene Names
KIAA1561
UniProt Entry Name
UACA_HUMAN
Uniprot Description
UACA: Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 15q22-q24
Cellular Component: cytoplasm; mitochondrion; nucleus
Molecular Function: protein binding
Research Articles on UACA
Similar Products
Product Notes
The UACA uaca (Catalog #AAA439765) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The UACA / Nucling (Nuclear Membrane Marker) Mouse Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UACA / Nucling can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1.0ug/ml) Western Blot (0.5-1ug/ml). Researchers should empirically determine the suitability of the UACA uaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UACA / Nucling, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
