Semaphorin-3A (N-terminal) Peptide | SEMA3A peptide

Semaphorin-3A (N-terminal) Peptide

Cat. Num. AAA474063

• Gene Names: SEMA3A; SemD; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243
• Applications: ELISA
• Synonyms: Semaphorin-3A (N-terminal) Peptide; SEMA3A peptide

• Specificity: The peptide is specifically recognized by anti-Sema3A (N-terminal) antibody (MBS472059) in ELISA, and has been shown to block the reactivity of (MBS472059) during Western blot. In addition, the peptide is recommended for use in blocking (MBS472059) reactivity in immunocytochemistry.
• Form/Format: Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
• Sequence Length: 771

• Applicable Applications for SEMA3A peptide: ELISA, Blocking
• Application Notes: Blocking: 1:1,000; ELISA: 50 ng/well; ; End user should determine optimal concentration dependent on the concentration of the antibody.; Recommended for blocking antibody reactivity in Western Blot and Immunocytochemistry, ELISA established in 96-well Nunc Immunoplates where peptide was bound to plates for 2 hours in 0.1 M sodium carbonate buffer, pH 8.5.
• Peptide Sequence: Semaphorin 3A synthetic peptide corresponds to amino acids in the Nterminal region of human Sema3A. This sequence is identical to similar regions in rat and mouse Sema3A, and has low homology to other semaphorin family members.
• Preparation and Storage: Store at -20°C. Stable for 1 year.

Related Product Information for SEMA3A peptide

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPIanchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin -1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

References

• Kolodokin, A.L. et al. (1993) Cell 75:1389.
• Luo, Y. et al. (1993) Cell 75:217.
• Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.

NCBI and Uniprot Product Information

• NCBI GI #: 5174673
• NCBI GeneID: 10371
• NCBI Accession #: NP_006071.1
• NCBI GenBank Nucleotide #: NM_006080.2
• UniProt Accession #: Q14563; Q75MQ2; Q86UJ2
• NCBI Official Full Name: semaphorin-3A
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• NCBI Official Symbol: SEMA3A
• NCBI Official Synonym Symbols: SemD; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243
• NCBI Protein Information: semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; OTTHUMP00000161379; OTTHUMP00000208884
• UniProt Protein Name: Semaphorin-3A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3A
• UniProt Synonym Gene Names: SEMAD
• UniProt Entry Name: SEM3A_HUMAN

NCBI Description

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq]

Uniprot Description

Function: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1

Subcellular location: Secreted

Domain: Strong binding to neuropilin is mediated by the carboxy third of the protein.

Sequence similarities: Belongs to the semaphorin family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 PSI domain.Contains 1 Sema domain.