Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CYBA Antibody.)
Rabbit anti-Human, Mouse CYBA Polyclonal Antibody | anti-CYBA antibody
CYBA Antibody
Gene Names
CYBA; p22-PHOX
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CYBA; Polyclonal Antibody; CYBA Antibody; Cytochrome b 245 alpha polypeptide; Cytochrome b 245 light chain; Cytochrome b alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b(558) alpha subunit; Cytochrome b558 subunit alpha; Flavocytochrome b 558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit; anti-CYBA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CYBA antibody detects endogenous levels of total CYBA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
195
Applicable Applications for anti-CYBA antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human CYBA
Subcellular Location
Cell Membrane
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CYBA Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CYBA Antibody.)
Western Blot (WB)
(Western blot analysis of CYBA using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis of CYBA using HeLa whole cell lysates)
Related Product Information for anti-CYBA antibody
Function: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Subunit Structure: Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9).
Post-translational Modifications: The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245. Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding. Ubiquitinated at Lys-149 likely by RNF145.
Similarity: Belongs to the p22phox family.
Subunit Structure: Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9).
Post-translational Modifications: The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245. Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding. Ubiquitinated at Lys-149 likely by RNF145.
Similarity: Belongs to the p22phox family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 21 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
cytochrome b-245 light chain
NCBI Official Synonym Full Names
cytochrome b-245 alpha chain
NCBI Official Symbol
CYBA
NCBI Official Synonym Symbols
p22-PHOX
NCBI Protein Information
cytochrome b-245 light chain
UniProt Protein Name
Cytochrome b-245 light chain
Protein Family
UniProt Gene Name
CYBA
UniProt Synonym Gene Names
p22phox
NCBI Description
Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]
Uniprot Description
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Research Articles on CYBA
Similar Products
Product Notes
The CYBA cyba (Catalog #AAA9602958) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYBA Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CYBA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the CYBA cyba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYBA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
