Testing Data
(Detection limit for recombinant GST tagged NEK4 is ~0.3ng/ml as a capture antibody.)
Mouse anti-Human NEK4 Monoclonal Antibody | anti-NEK4 antibody
NEK4 (Never in Mitosis A-related Kinase 4, NimA-related Protein Kinase 4, NRK2, pp12301, Serine/threonine Protein Kinase 2, STK2, Serine/threonine Protein Kinase Nek4, Serine/threonine Protein Kinase NRK2, MGC33171) (Biotin)
Gene Names
NEK4; NRK2; STK2; pp12301
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NEK4; Monoclonal Antibody; NEK4 (Never in Mitosis A-related Kinase 4; NimA-related Protein Kinase 4; NRK2; pp12301; Serine/threonine Protein Kinase 2; STK2; Serine/threonine Protein Kinase Nek4; Serine/threonine Protein Kinase NRK2; MGC33171) (Biotin); anti-NEK4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1B8
Specificity
Recognizes human NEK4.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-NEK4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa680-781 from human NEK4 (AAH63044) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
DVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYRIF
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Detection limit for recombinant GST tagged NEK4 is ~0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged NEK4 is ~0.3ng/ml as a capture antibody.)
Related Product Information for anti-NEK4 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-NEK4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
160.0 kDa
NCBI Official Full Name
Homo sapiens NIMA (never in mitosis gene a)-related kinase 4, mRNA
NCBI Official Synonym Full Names
NIMA related kinase 4
NCBI Official Symbol
NEK4
NCBI Official Synonym Symbols
NRK2; STK2; pp12301
NCBI Protein Information
serine/threonine-protein kinase Nek4
Protein Family
NCBI Description
The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017]
Research Articles on NEK4
Similar Products
Product Notes
The NEK4 (Catalog #AAA6143145) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NEK4 (Never in Mitosis A-related Kinase 4, NimA-related Protein Kinase 4, NRK2, pp12301, Serine/threonine Protein Kinase 2, STK2, Serine/threonine Protein Kinase Nek4, Serine/threonine Protein Kinase NRK2, MGC33171) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NEK4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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