phospho-PKCa (Ser-657/Tyr-658) Peptide | PRKCA peptide

phospho-PKC alpha (Ser-657/Tyr-658) Peptide

Cat. Num. AAA474052

• Gene Names: PRKCA; AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha
• Synonyms: phospho-PKCa (Ser-657/Tyr-658) Peptide; phospho-PKC alpha (Ser-657/Tyr-658) Peptide; PRKCA peptide

• Specificity: The peptide is specifically recognized by anti-PKC-alpha (Ser-657/Tyr-658) phospho-specific antibody (PP1091) in ELISA, and has been shown to block the reactivity of PP1091 during Western blot. In addition, the peptide is recommended for use in blocking PP1091 reactivity in immunocytochemistry.
• Form/Format: Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
• Sequence: Peptide Sequence: Phospho-PKCalpha (Ser-657/Tyr-658) synthetic peptide corresponds to amino acid residues around serine 657 and tyrosine 658 of human PKCalpha. This sequence is similar to a dual phosphorylation site found in PKC-beta-I (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKC-alpha and PKC-betaI. In addition, this dual site is homologous to threonine 674 and tyrosine 675 in human PKC-gamma, although this blocking activity has not been confirmed.

• Application Notes: Antibody Blocking: 1 ug/ml; ELISA: 10-100 ng/well
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Related Product Information for PRKCA peptide

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (alpha, beta-I, beta-II, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, Fc-epsilon-RI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKC-beta-I and PKC-alpha, respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

References

• Kawakami et al. (2003) Proc. Natl. Acad. Sci. USA 100:9470-9475.
• Nishizuka, Y. (1988) Nature 334:661.

NCBI and Uniprot Product Information

• NCBI GI #: 4506067
• NCBI GeneID: 5578
• NCBI Accession #: NP_002728.1
• NCBI GenBank Nucleotide #: NM_002737.2
• UniProt Accession #: P17252; Q15137; Q32M72; Q96RE4; Q2TSD3; Q59FI5; Q7Z727
• Molecular Weight: 76,750 Da
• NCBI Official Full Name: protein kinase C alpha type
• NCBI Official Synonym Full Names: protein kinase C, alpha
• NCBI Official Symbol: PRKCA
• NCBI Official Synonym Symbols: AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha
• NCBI Protein Information: protein kinase C alpha type; PKC-A; aging-associated gene 6
• UniProt Protein Name: Protein kinase C alpha type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCA
• UniProt Synonym Gene Names: PKCA; PRKACA
• UniProt Entry Name: KPCA_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]

Uniprot Description

Function: This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. Ref.9PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Ref.9

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain

Subunit structure: Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and GNB2L1/RACK1

By similarity. Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine. Interacts with PICK1 (via PDZ domain). Interacts with TRIM41. Ref.7 Ref.8 Ref.9 Ref.13

Subcellular location: Cytoplasm

By similarity. Cell membrane; Peripheral membrane protein

By similarity. Nucleus

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 C2 domain.Contains 2 phorbol-ester/DAG-type zinc fingers.Contains 1 protein kinase domain.