Western Blot (WB)
(Anti- PKC alpha Picoband antibody, MBS178350, Western blottingAll lanes: Anti PKC alpha (MBS178350) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: Mouse Cardiac Muscle Tissue Lysate at 50ugLane 4: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 77KDObserved bind size: 77KD)
PKC alpha Polyclonal Antibody | anti-PKC alpha antibody
Anti-PKC alpha Antibody
Western Blot (WB)
(Anti- PKC alpha Picoband antibody, MBS178350, Western blottingAll lanes: Anti PKC alpha (MBS178350) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: Mouse Cardiac Muscle Tissue Lysate at 50ugLane 4: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 77KDObserved bind size: 77KD)
Western Blot (WB)
(Anti- PKC alpha Picoband antibody, MBS178350, Western blottingAll lanes: Anti PKC alpha (MBS178350) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: Mouse Cardiac Muscle Tissue Lysate at 50ugLane 4: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 77KDObserved bind size: 77KD)
Background: Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PRKCA1 regulates cardiac contractility and propensity toward heart failure.
NCBI and Uniprot Product Information
NCBI Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.
Protein type: Oncoprotein; Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; PKC family; Alpha subfamily
Chromosomal Location of Human Ortholog: 17q22-q23.2
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; mitochondrial membrane; mitochondrion; nucleoplasm; perinuclear region of cytoplasm; plasma membrane
Molecular Function: ATP binding; calcium-dependent protein kinase C activity; enzyme binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity; zinc ion binding
Biological Process: adenylate cyclase activation; angiogenesis; cell adhesion; mitotic nuclear envelope disassembly; negative regulation of adenylate cyclase activity; peptidyl-serine phosphorylation; platelet activation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of macrophage differentiation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of insulin secretion; regulation of mRNA stability
Research Articles on PKC alpha
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Product Notes
The PKC alpha prkca (Catalog #AAA178350) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKC alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKC alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the PKC alpha prkca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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