phospho-PAK6 (Ser-165) Peptide | PAK6 peptide

phospho-PAK6 (Ser-165) Blocking Peptide

Cat. Num. AAA474048

• Gene Names: PAK6; PAK5
• Synonyms: phospho-PAK6 (Ser-165) Peptide; phospho-PAK6 (Ser-165) Blocking Peptide; PAK6 peptide

• Specificity: The peptide is specifically recognized by anti-PAK6 (Ser-165) phospho-specific antibody (PP1551) in ELISA, and has been shown to block the reactivity of PP1551 during Western blot. In addition, the peptide is recommended for use in blocking PP1551 reactivity in immunocytochemistry.
• Form/Format: Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
• Sequence: Peptide Sequence: PAK6 (Ser-165) synthetic peptide corresponds to amino acids around serine 165 of human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.

• Application Notes: Antibody Blocking: 1 ug/ml; ELISA: 10-100 ng/well
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Testing Data

Related Product Information for PAK6 peptide

p21-activated kinase 6 (PAK6) is a member of the PAK family of serine/threonine kinases. These kinases have a highly conserved amino-terminal Cdc42/Rac interactive binding domain and a carboxylterminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 interacts with the androgen receptor, a steroid hormone transcription factor involved in male sexual differentiation and development. PAK6 is highly expressed in testis and prostate tissues. Regulation of PAK6 kinase activity occurs through multiple sites of phosphorylation. Activation of PAK6 requires autophosphorylation of Ser-560 and MKK-6 induced phosphorylation of Tyr-566. In addition, p38 MAPK can phosphorylate Ser-165, which increases PAK6 kinase activity. Thus, multiple signaling pathways may regulate the activity of PAK6 through differential phosphorylation.

References

• Kaur, R. et al. (2005) J. Biol. Chem. 280(5) :3323.
• Schrantz, N. et al. (2004) J. Biol. Chem. 279(3):1922.
• Lee, S.R. et al. (2002) Mol. Endocrin. 16(1):85.

NCBI and Uniprot Product Information

• NCBI GI #: 23272546
• NCBI GeneID: 56924
• UniProt Accession #: Q9NQU5
• Molecular Weight: 74,869 Da
• NCBI Official Full Name: PAK6 protein
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 6
• NCBI Official Symbol: PAK6
• NCBI Official Synonym Symbols: PAK5
• NCBI Protein Information: serine/threonine-protein kinase PAK 6; PAK-5; PAK-6; OTTHUMP00000160372; p21-activated kinase 6; p21(CDKN1A)-activated kinase 6; p21-activated protein kinase 6
• UniProt Protein Name: Serine/threonine-protein kinase PAK 6
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK6
• UniProt Synonym Gene Names: PAK5
• UniProt Entry Name: PAK6_HUMAN

NCBI Description

This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: The activated kinase acts on a variety of targets

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1

By similarity. Interacts with the androgen receptor.

Post-translational modification: Autophosphorylated when activated by CDC42/p21

By similarity. Ref.4 Ref.5 Ref.6

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 CRIB domain.Contains 1 protein kinase domain.