Blasticidin S Deaminase Peptide
Blasticidin S Deaminase Blocking Peptide
Synonyms
Blasticidin S Deaminase Peptide; Blasticidin S Deaminase Blocking Peptide; Blasticidin S Deaminase Peptide peptide
Specificity
The peptide is specifically recognized by anti-Blasticidin S Deaminase antibody (BP1231) in ELISA, and is recommended for use in blocking BP1231 reactivity in Western blot and immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: A synthetic peptide corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.
Application Notes
Antibody Blocking: 1 ug/ml
ELISA: 10-100 ng/well
ELISA: 10-100 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Related Product Information for Blasticidin S Deaminase Peptide peptide
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week. Catalog# BP1231 is a Bsd antibody that can detect Bsd expression in cells transfected with blasticidin-selectable constructs. This antibody is useful for detection of successful gene transfer in constructs that utilize blasticidin selection.
References
• Kimura M, et al. (1994). Biochim Biophys Acta. 1219(3):653-9.
• Izumi M, et al. (1991). Exp Cell Res. 197(2):229-33.
• Izumi M, et al. (1991). Exp Cell Res. 197(2):229-33.
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Product Notes
The Blasticidin S Deaminase Peptide (Catalog #AAA474004) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Antibody Blocking: 1 ug/ml ELISA: 10-100 ng/well. Researchers should empirically determine the suitability of the Blasticidin S Deaminase Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: A synthetic peptide correspond ing to amino acid residues in the N-terminal region of Blasticidi n S Deaminase from Aspergillu s Terrus. It is sometimes possible for the material contained within the vial of "Blasticidin S Deaminase Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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