Histone Deacetylase, Assay Developer Peptide Substrate
Histone Deacetylase, Assay Developer (2X) (HDAC)
Synonyms
Histone Deacetylase; Assay Developer; Assay Developer (2X) (HDAC); Assay Developer peptide substrate
Form/Format
Supplied as a liquid (2X Concentrate).
Application Notes
Study of HDAC or sirtuin kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications. Cell-based HDAC assays.
Preparation and Storage
-70 degree C. Store, long term, only as the 2X concentrate, at -70 degree C. Concentrate is stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing). Frozen storage of smaller aliquots is recommended.
Related Product Information for Histone Deacetylase, Assay Developer peptide substrate
HDAC assay developer is an essential reagent for signal generation in HDAC or sirtuin assays done with any of HDAC fluorimetric substrates. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Typically, an 'inhibitor stop' will be included in the HDAC developer (e.g.Trichostatin A for class I and II HDACs, nicotinamide for sirtuins).
Product Categories/Family for Histone Deacetylase, Assay Developer peptide substrate
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Product Notes
The Histone Deacetylase, Assay Developer (Catalog #AAA634024) is a Peptide Substrate and is intended for research purposes only. The product is available for immediate purchase. Study of HDAC or sirtuin kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications. Cell-based HDAC assays. Researchers should empirically determine the suitability of the Histone Deacetylase, Assay Developer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone Deacetylase, Assay Developer, Peptide Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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