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Typical Testing Data/Standard Curve (for reference only)

Human peptidase D ELISA Kit | PEPD elisa kit

Human Xaa-Pro Dipeptidase/Prolidase, PEPD ELISA Kit

Gene Names
PEPD; PROLIDASE
Reactivity
Human
Synonyms
peptidase D; Human Xaa-Pro Dipeptidase/Prolidase; PEPD ELISA Kit; Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit; MGC10905; PROLIDASE; aminoacyl-L-proline hydrolase; imidodipeptidase; prolidase; proline dipeptidase; PEPD elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PEPD. No significant cross-reactivity or interference between Human PEPD and analogues was observed.
Sequence Length
493
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
93.75 mU/ml-6000 mU/ml
Sensitivity
23.4 mU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Developmental Protein
Protein Biological Process 3
Collagen degradation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PEPD elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PEPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEPD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEPD bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,548 Da
NCBI Official Full Name
xaa-Pro dipeptidase isoform 1
NCBI Official Synonym Full Names
peptidase D
NCBI Official Symbol
PEPD
NCBI Official Synonym Symbols
PROLIDASE
NCBI Protein Information
xaa-Pro dipeptidase; imidodipeptidase; X-Pro dipeptidase; proline dipeptidase; aminoacyl-L-proline hydrolase
UniProt Protein Name
Xaa-Pro dipeptidase
Protein Family
UniProt Gene Name
PEPD
UniProt Synonym Gene Names
PRD; X-Pro dipeptidase; Prolidase
UniProt Entry Name
PEPD_HUMAN

NCBI Description

This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

Function: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.

Catalytic activity: Hydrolysis of Xaa-|-Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.

Cofactor: Binds 2 manganese ions per subunit.1 PublicationManual assertion based on experiment in:Ref.10

Research Articles on PEPD

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Product Notes

The Human PEPD pepd (Catalog #AAA705782) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA705782 ELISA Kit recognizes Human PEPD. It is sometimes possible for the material contained within the vial of "peptidase D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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