Typical Testing Data/Standard Curve (for reference only)
Human Peptidase D (PEPD) ELISA Kit | PEPD elisa kit
Mini Samples Peptidase D (PEPD) ELISA Kit
Gene Names
PEPD; PROLIDASE
Reactivity
Human
Applications
ELISA
Synonyms
Peptidase D (PEPD); Mini Samples Peptidase D (PEPD) ELISA Kit; PRD; Xaa-Pro Dipeptidase; Prolidase; Imidodipeptidase; Proline dipeptidase; PEPD elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PEPD. No significant cross-reactivity or interference between PEPD and analogues was observed.
Applicable Applications for PEPD elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Human tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.064ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PEPD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PEPD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PEPD elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PEPD in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PEPD. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PEPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PEPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PEPD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PEPD. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PEPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PEPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PEPD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,261 Da
NCBI Official Full Name
xaa-Pro dipeptidase isoform 1
NCBI Official Synonym Full Names
peptidase D
NCBI Official Symbol
PEPD
NCBI Official Synonym Symbols
PROLIDASE
NCBI Protein Information
xaa-Pro dipeptidase
UniProt Protein Name
Xaa-Pro dipeptidase
Protein Family
UniProt Gene Name
PEPD
UniProt Synonym Gene Names
PRD; X-Pro dipeptidase; Prolidase
UniProt Entry Name
PEPD_HUMAN
Uniprot Description
peptidase D: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.13.9; Protease
Chromosomal Location of Human Ortholog: 19q13.11
Molecular Function: metallocarboxypeptidase activity; protein binding
Biological Process: amino acid metabolic process
Disease: Prolidase Deficiency
Similar Products
Product Notes
The Human PEPD pepd (Catalog #AAA2709427) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709427 ELISA Kit recognizes Human PEPD. AAA Biotech's Peptidase D (PEPD) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the PEPD pepd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peptidase D (PEPD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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