SDS-PAGE
50S ribosomal protein L32 Recombinant Protein | L32 recombinant protein
Recombinant E Coli 50S ribosomal protein L32
Gene Names
rpmF; ECK1075; JW1075
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
50S ribosomal protein L32; Recombinant E Coli 50S ribosomal protein L32; L32 recombinant protein
Host
E Coli
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
2-57aa; Full Length
Sequence
AVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK
Sequence Length
57
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
References
Cloning and analysis of an Escherichia coli operon containing the rpmF gene for ribosomal protein L32 and the gene for a 30-kilodalton protein.Tanaka Y., Tsujimura A., Fujita N., Isono S., Isono K.J. Bacteriol. 171:5707-5712(1989)
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.DNA Res. 3:137-155(1996)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
The primary structure of protein L32 from the 50S subunit of Escherichia coli ribosomes.Wittmann-Liebold B., Greuer B., Pannenbecker R.Hoppe-Seyler's Z. Physiol. Chem. 356:1977-1979(1975)
The primary structure of ribosomal protein L32 from E. coli MRE-600 ribosomes.Vinokurov L.M., Alakhov Y.B., Golov E.A., Ovchinnikov Y.A.Bioorg. Khim. 2:1013-1017(1976)
Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli.Walleczek J., Martin T., Redl B., Stoeffler-Meilicke M., Stoeffler G.Biochemistry 28:4099-4105(1989)
Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997)
Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.Arnold R.J., Reilly J.P.Anal. Biochem. 269:105-112(1999)
Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement.Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.Cell 113:789-801(2003)
Structures of the bacterial ribosome at 3.5 A resolution.Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.Science 310:827-834(2005)
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
33.3 kDa
NCBI Official Symbol
rpmF
NCBI Official Synonym Symbols
ECK1075; JW1075
NCBI Protein Information
50S ribosomal subunit protein L32
UniProt Protein Name
50S ribosomal protein L32
Protein Family
UniProt Gene Name
rpmF
UniProt Entry Name
RL32_ECOLI
NCBI Description
The L32 protein is a component of the 50S subunit of the ribosome. [More information is available at EcoCyc: EG10890].
Research Articles on L32
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Product Notes
The L32 rpmf (Catalog #AAA717184) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-57aa; Full Length. The amino acid sequence is listed below: AVQQNKPTRS KRGMRRSHDA LTAVTSLSVD KTSGEKHLRH HITADGYYRG RKVIAK. It is sometimes possible for the material contained within the vial of "50S ribosomal protein L32, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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