Mouse Pyridoxal phosphate phosphatase ELISA Kit | Pdxp elisa kit

Mouse Pyridoxal phosphate phosphatase ELISA Kit

Cat. Num. AAA2884633

• Gene Names: Pdxp; PLPP; AB041662; 1600027H05Rik
• Reactivity: Mouse
• Synonyms: Pyridoxal phosphate phosphatase; Mouse Pyridoxal phosphate phosphatase ELISA Kit; PLP phosphatase; Chronophin; Pdxp; Cin; Plp; Plpp; 3.1.3.3; Pdxp elisa kit

• Reactivity: Mouse
• Sequence Length: 292

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Pdxp elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47059486
• NCBI GeneID: 57028
• NCBI Accession #: NP_064667.2
• NCBI GenBank Nucleotide #: NM_020271.3
• UniProt Accession #: P60487
• Molecular Weight: 31,512 Da
• NCBI Official Full Name: pyridoxal phosphate phosphatase
• NCBI Official Synonym Full Names: pyridoxal (pyridoxine, vitamin B6) phosphatase
• NCBI Official Symbol: Pdxp
• NCBI Official Synonym Symbols: PLPP; AB041662; 1600027H05Rik
• NCBI Protein Information: pyridoxal phosphate phosphatase
• UniProt Protein Name: Pyridoxal phosphate phosphatase
• Protein Family: Pyridoxal phosphate phosphatase
• UniProt Gene Name: Pdxp
• UniProt Synonym Gene Names: Cin; Plp; Plpp; PLP phosphatase
• UniProt Entry Name: PLPP_MOUSE

Uniprot Description

PDXP: Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho- tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP. Homodimer. Ubiquitous. Highly expressed in all the regions of central nerve system except the spinal cord. Also expressed at high level in liver and testis. In fetus, it is weakly expressed in all organs except brain. Inhibited by NaF, Zn(2+), Ca(2+), Mn(2+) and EDTA. Belongs to the HAD-like hydrolase superfamily.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.3; Cofactor and Vitamin Metabolism - vitamin B6; Cell cycle regulation; EC 3.1.3.74

Cellular Component: actin cytoskeleton; cleavage furrow; cytosol; intercellular junction; lamellipodium; midbody; plasma membrane

Molecular Function: growth factor binding; heat shock protein binding; phosphoprotein phosphatase activity

Biological Process: actin rod formation; positive regulation of actin filament depolymerization; protein amino acid dephosphorylation; regulation of cytokinesis; regulation of mitosis