Human Pyridoxal phosphate phosphatase ELISA Kit | PDXP elisa kit

Human Pyridoxal phosphate phosphatase ELISA Kit

Cat. Num. AAA763231

• Gene Names: PDXP; CIN; PLP; dJ37E16.5
• Reactivity: Human
• Synonyms: Pyridoxal phosphate phosphatase; Human Pyridoxal phosphate phosphatase ELISA Kit; PDXP/Pyridoxal phosphate phosphatase/PLP phosphatase/Chronophin/CIN/PLP/PLPP; PDXP elisa kit

• Reactivity: Human
• Sequence Length: 296

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PDXP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PDXP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDXP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDXP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDXP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10092677
• NCBI GeneID: 57026
• NCBI Accession #: NP_064711.1
• NCBI GenBank Nucleotide #: NM_020315.4
• Molecular Weight: 31,698 Da
• NCBI Official Full Name: pyridoxal phosphate phosphatase
• NCBI Official Synonym Full Names: pyridoxal phosphatase
• NCBI Official Symbol: PDXP
• NCBI Official Synonym Symbols: CIN; PLP; dJ37E16.5
• NCBI Protein Information: pyridoxal phosphate phosphatase
• UniProt Protein Name: Pyridoxal phosphate phosphatase
• Protein Family: Pyridoxal phosphate phosphatase
• UniProt Gene Name: PDXP
• UniProt Synonym Gene Names: CIN; PLP; PLPP; PLP phosphatase
• UniProt Entry Name: PLPP_HUMAN

NCBI Description

Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP (Jang et al., 2003 [PubMed 14522954]).[supplied by OMIM, Mar 2008]

Uniprot Description

PDXP: Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho- tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP. Homodimer. Ubiquitous. Highly expressed in all the regions of central nerve system except the spinal cord. Also expressed at high level in liver and testis. In fetus, it is weakly expressed in all organs except brain. Inhibited by NaF, Zn(2+), Ca(2+), Mn(2+) and EDTA. Belongs to the HAD-like hydrolase superfamily.

Protein type: Cell cycle regulation; Cofactor and Vitamin Metabolism - vitamin B6; EC 3.1.3.74; EC 3.1.3.3; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: actin cytoskeleton; cleavage furrow; cytosol; lamellipodium; midbody; plasma membrane

Molecular Function: heat shock protein binding; magnesium ion binding; phosphoprotein phosphatase activity; phosphoserine phosphatase activity; protein binding; pyridoxal phosphatase activity

Biological Process: actin rod formation; positive regulation of actin filament depolymerization; protein amino acid dephosphorylation; pyridoxal phosphate catabolic process; regulation of cytokinesis; regulation of mitosis