Typical Testing Data/Standard Curve (for reference only)
Canine Programmed cell death 1 ligand 1 ELISA Kit | PDL1/B7-H1/CD274 elisa kit
Canine Programmed cell death 1 ligand 1 ELISA Kit
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Canine
Synonyms
Programmed cell death 1 ligand 1; Canine Programmed cell death 1 ligand 1 ELISA Kit; PDL1/B7-H1/CD274 elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of PCD1L1. No significant cross-reactivity or interference between PCD1L1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCD1L1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PDL1/B7-H1/CD274 elisa kit
Intended Uses: This PCD1L1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of CaninePCD1L1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: PCD1L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCD1L1 antibody and an PCD1L1-HRP conjugate. The assay sample and buffer are incubated together with PCD1L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCD1L1 concentration since PCD1L1 from samples and PCD1L1-HRP conjugate compete for the anti-PCD1L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PCD1L1 from the sample, fewer sites are left to bind PCD1L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCD1L1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PCD1L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCD1L1 antibody and an PCD1L1-HRP conjugate. The assay sample and buffer are incubated together with PCD1L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCD1L1 concentration since PCD1L1 from samples and PCD1L1-HRP conjugate compete for the anti-PCD1L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PCD1L1 from the sample, fewer sites are left to bind PCD1L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCD1L1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,454 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform a
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
Protein Family
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
Uniprot Description
CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p24
Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane
Molecular Function: protein binding
Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction
Research Articles on PDL1/B7-H1/CD274
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Product Notes
The Canine PDL1/B7-H1/CD274 cd274 (Catalog #AAA746693) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746693 ELISA Kit recognizes Canine PDL1/B7-H1/CD274. It is sometimes possible for the material contained within the vial of "Programmed cell death 1 ligand 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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