Pyruvate Dehydrogenase Kinase Isozyme 1 Recombinant Protein | PDK1 recombinant protein

Recombinant Human Pyruvate Dehydrogenase Kinase Isozyme 1

Cat. Num. AAA143676

• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Pyruvate Dehydrogenase Kinase Isozyme 1; Recombinant Human Pyruvate Dehydrogenase Kinase Isozyme 1; PDK1 Human; Pyruvate Dehydrogenase Kinase Isozyme 1 Human Recombinant; Pyruvate dehydrogenase kinase isoform 1; PDK1; Pyruvate dehydrogenase (lipoamide) kinase isozyme 1 mitochondrial; PDK1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: PDK1 solution containing 20mM Tris pH-7, 0.5mM DTT, 0.1M NaCl, 0.1mM EDTA 0.1mM PMSF, 1mM MgCl2 and 40% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSSDSGSSPA SERGVPGQVD FYARFSPSPL SMKQFLDFGS VNACEKTSFM FLRQELPVRL ANIMKEISLLPDNLLRTPSV QLVQSWYIQS LQELLDFKDK SAEDAKAIYD FTDTVIRIRN RHNDVIPTMA QGVIEYKESF GVDPVTSQNV QYFLDRFYMS RISIRMLLNQ HSLLFGGKGK GSPSHRKHIG SINPNCNVLE VIKDGYENAR RLCDLYYINS PELELEELNA KSPGQPIQVV YVPSHLYHMVFELFKNAMRA TMEHHANRGV YPPIQVHVTL GNEDLTVKMS DRGGGVPLRK IDRLFNYMYS TAPRPRVETS RAVPLAGFGY GLPISRLYAQ YFQGDLKLYS LEGYGTDAVI YIKALSTDSI ERLPVYNKAA WKHYNTNHEA DDWCVPSREP KDMTTFRSA
• Sequence Length: 456

• Preparation and Storage: PDK1 Human Recombinant although stable at 4 degree C for 1 week, should be stored below -18 degree C. Please prevent freeze thaw cycles.

Related Product Information for PDK1 recombinant protein

PDK1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 429 amino acids (29-436) and having a molecular mass of 48.6 kDa. PDK1 is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for PDK1 recombinant protein

Protein Kinases; Enzymes; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 514052656
• NCBI GeneID: 5163
• NCBI Accession #: NP_001265478.1
• NCBI GenBank Nucleotide #: NM_001278549.1
• UniProt Accession #: Q15118; Q308M4; B2R6T1; B7Z937; D3DPD8; E9PD65
• NCBI Official Full Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 1
• NCBI Official Symbol: PDK1
• NCBI Protein Information: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; PDH kinase 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; pyruvate dehydrogenase kinase, isoenzyme 1
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: PDK1
• UniProt Synonym Gene Names: PDHK1; PDH kinase 1
• UniProt Entry Name: PDK1_HUMAN

NCBI Description

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

PDHK1: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK1 is detected in heart, pancreatic islets, and skeletal muscles. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. PDHK1 is a direct HIF-1 target gene. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA) and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Kinase, protein; EC 2.7.11.2; Mitochondrial; Protein kinase, atypical; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial pyruvate dehydrogenase complex

Molecular Function: protein binding; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; cellular metabolic process; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; pyruvate metabolic process; protein amino acid phosphorylation; induction of apoptosis by oxidative stress