PDE1B recombinant protein

Recombinant Human PDE1B Protein (His & GST tag)

Gene Names

PDE1B; PDE1B1; PDES1B; HEL-S-79p

Purity

> 94 % as determined by SDS-PAGE

Synonyms

PDE1B; Recombinant Human PDE1B Protein (His & GST tag); PDE1B1; PDES1B; PDE1B recombinant protein

Ordering
Cat. Num. AAA2545679 For Research Use Only!

Host

Baculovirus-Insect Cells

Purity/Purification

> 94 % as determined by SDS-PAGE

Form/Format

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0

Sequence Length

536

Application Notes

The recombinant human PDE1B/GST chimera consists of 773 amino acids and has a calculated molecular mass of 89.2 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.

Predicted N Terminal

Met

Endotoxin

< 1.0 EU per mug of the protein as determined by the LAL method

Preparation and Storage

Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for PDE1B recombinant protein

Background: Calcium/calmodulin-dependent 3', 5'-cyclic nucleotide phosphodiesterase 1B, also known as Cam-PDE 1B and PDE1B, is a cytoplasm protein which belongs to the cyclic nucleotide phosphodiesterase family and PDE1 subfamily. Phosphodiesterase-10A (PDE10A), Phosphodiesterase-1B (PDE1B), Phosphodiesterase-4B (PDE4B), and Phosphodiesterase-4A (PDE4A) are important regulators of signal transduction in striatum due to their catalysis of cyclic AMP and cyclic GMP. PDE1B is highly expressed in the striatum. It binds two divalent metal cations per subunit. Site one of PDE1B may preferentially bind zinc ions, while site two of PDE1B has a preference for magnesium and/or manganese ions. PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. It has a preference for cGMP as a substrate.

Description: A DNA sequence encoding the human PDE1B long isoform (Q01064-1) (Met 1-Asp 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

NCBI GI #

4505677

NCBI GeneID

5153

NCBI Accession #

NP_000915.1

NCBI GenBank Nucleotide #

NM_000924.3

UniProt Accession #

Q01064; Q92825; Q96KP3

Molecular Weight

59,051 Da

NCBI Official Full Name

calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1

NCBI Official Synonym Full Names

phosphodiesterase 1B

NCBI Official Symbol

PDE1B

NCBI Official Synonym Symbols

PDE1B1; PDES1B; HEL-S-79p

NCBI Protein Information

calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

UniProt Protein Name

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

UniProt Gene Name

PDE1B

UniProt Synonym Gene Names

PDE1B1; PDES1B; Cam-PDE 1B

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; protein binding

Biological Process: apoptosis; cAMP catabolic process; cGMP catabolic process; monocyte differentiation

Research Articles on PDE1B

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

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Product Notes

The PDE1B pde1b (Catalog #AAA2545679) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human PDE1B/GST chimera consists of 773 amino acids and has a calculated molecular mass of 89.2 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the PDE1B pde1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE1B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.