Rabbit anti-Human, Mouse NALP4 Polyclonal Antibody | anti-NLRP4 antibody

NALP4 Antibody

Cat. Num. AAA150202

• Gene Names: NLRP4; CT58; PAN2; RNH2; NALP4; PYPAF4; CLR19.5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: NALP4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: NALP4; Polyclonal Antibody; NALP4 Antibody; CT58; PAN2; RNH2; PYPAF4; CLR19.5; NACHT; LRR and PYD domains-containing protein 4; PAAD and NACHT-containing protein 2; NLR family; pyrin domain containing 4; anti-NLRP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NALP4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; NALP4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 994

• Applicable Applications for anti-NLRP4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: NALP4 antibody can be used for detection of NALP4 by Western blot at 1 ug/mL.; Antibody validated: Western Blot in human samples.; ; All other applications and species not yet tested.; Optimal dilutions for each application to be determined by the researcher
• Conjugate: Unconjugated
• Immunogen: NALP4 antibody was raised against a 13 amino acid synthetic peptide near the center of human NALP4.; The immunogen is located within amino acids 330 - 380 of NALP4.
• Positive Control: MBS151739 Cell Lysate
• Preparation and Storage: NALP4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NALP4 in K562 cell lysate with NALP4 antibody at 1 μg/mL.)

Related Product Information for anti-NLRP4 antibody

NALP4 Antibody: NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP4, also known as PYRIN-containing APAF1-like protein 4, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. In transfected 293 cells, NALP4 suppressed NF-κB induction by TNF-α and IL-1β, suggesting NALP4 operates at a point of convergence in these two signaling pathways. NALP4 also suppressed NF-κB induction resulting from overexpression of several adapter proteins and protein kinases involved in these pathways, including TRAF2, TRAF6, RIP, and IRAK2, as well as the IKK-alpha and IKK-beta, suggesting that NALP4 is critical in modulating NF-κB activity.

NCBI and Uniprot Product Information

• NCBI GI #: 17064172
• NCBI GeneID: 147945
• UniProt Accession #: Q96MN2; Q86W87; Q96AY6
• NCBI Official Full Name: NALP4
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 4
• NCBI Official Symbol: NLRP4
• NCBI Official Synonym Symbols: CT58; PAN2; RNH2; NALP4; PYPAF4; CLR19.5
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 4; cancer/testis antigen 58; ribonuclease inhibitor 2; PAAD and NACHT-containing protein 2; PYRIN and NACHT-containing protein 2; PYRIN-containing APAF1-like protein 4; NACHT, LRR and PYD containing protein 4; NACHT, leucine rich repeat and PYD containing 4; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 4
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 4
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP4
• UniProt Synonym Gene Names: NALP4; PAN2; PYPAF4; RNH2; CT58; PAN2; PYPAF4
• UniProt Entry Name: NALP4_HUMAN

NCBI Description

NALPs are cytoplasmic proteins that form a subfamily within the larger CATERPILLER protein family. Most short NALPs, such as NALP4, have an N-terminal pyrin (MEFV; MIM 608107) domain (PYD), followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. The long NALP, NALP1 (MIM 606636), also has a C-terminal extension containing a function to find domain (FIIND) and a caspase recruitment domain (CARD). NALPs are implicated in the activation of proinflammatory caspases (e.g., CASP1; MIM 147678) via their involvement in multiprotein complexes called inflammasomes (Tschopp et al., 2003 [PubMed 12563287]).[supplied by OMIM, Mar 2008]

Uniprot Description

NALP4: May be involved in inflammation. Acts as a negative regulator of the type I interferon signaling pathway by serving as an adapter to promote DTX4-mediated ubiquitination of activated TBK1, and its subsequent degradation. Belongs to the NLRP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: cytosol

Molecular Function: ATP binding

Biological Process: regulation of interferon type I production; positive regulation of interferon type I production; innate immune response; inflammatory response