Mouse FK506 Binding Protein 5 (FKBP5) ELISA Kit | FKBP5 elisa kit

Mouse FK506 Binding Protein 5 (FKBP5) ELISA Kit

Cat. Num. AAA2098065

• Gene Names: Fkbp5; Dit1; FKBP-5; FKBP51; D17Ertd592e
• Reactivity: Mouse
• Synonyms: FK506 Binding Protein 5 (FKBP5); Mouse FK506 Binding Protein 5 (FKBP5) ELISA Kit; P54; FKBP51; FKBP54; Ptg-10; Rotamase; 51 kDa FK506-binding protein; 54 kDa progesterone receptor-associated; Androgen-regulated protein 6; HSP90-binding immunophilin; FKBP5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 5 (FKBP5).; No significant cross-reactivity or interference between FK506 Binding Protein 5 (FKBP5) and analogues was observed.
• Sequence Length: 456

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.135ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 5 (FKBP5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 5 (FKBP5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FKBP5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 5 (FKBP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 5 (FKBP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 5 (FKBP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 5 (FKBP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FKBP5 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 6753884
• NCBI GeneID: 14229
• NCBI Accession #: NP_034350.1
• NCBI GenBank Nucleotide #: NM_010220.4
• UniProt Accession #: Q64378
• Molecular Weight: 50,966 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP5
• NCBI Official Synonym Full Names: FK506 binding protein 5
• NCBI Official Symbol: Fkbp5
• NCBI Official Synonym Symbols: Dit1; FKBP-5; FKBP51; D17Ertd592e
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP5
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP5
• Protein Family: FK506-binding protein
• UniProt Gene Name: Fkbp5
• UniProt Synonym Gene Names: Fkbp51; PPIase FKBP5; 51 kDa FKBP; FKBP-51; FKBP-5

Uniprot Description

Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded.