Rat Podocin ELISA Kit | PDCN elisa kit

Rat Podocin ELISA Kit

Cat. Num. AAA727017

• Gene Names: NPHS2; PDCN; SRN1
• Reactivity: Rat
• Synonyms: Podocin; Rat Podocin ELISA Kit; PDCN elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This PDCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PDCN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDCN elisa kit

Principle of the Assay: PDCN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDCN. Standards or samples are then added to the microtiter plate wells and PDCN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PDCN present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PDCN are added to each well to "sandwich" the PDCN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PDCN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDCN concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7657615
• NCBI GeneID: 7827
• NCBI Accession #: NP_055440.1
• NCBI GenBank Nucleotide #: NM_014625.3
• Molecular Weight: 34,421 Da
• NCBI Official Full Name: podocin isoform 1
• NCBI Official Synonym Full Names: nephrosis 2, idiopathic, steroid-resistant (podocin)
• NCBI Official Symbol: NPHS2
• NCBI Official Synonym Symbols: PDCN; SRN1
• NCBI Protein Information: podocin
• UniProt Protein Name: Podocin
• Protein Family: Podocin
• UniProt Gene Name: NPHS2
• UniProt Entry Name: PODO_HUMAN

NCBI Description

This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: protein complex; integral to plasma membrane; endoplasmic reticulum; plasma membrane; intercellular junction; lipid raft

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; excretion

Disease: Nephrotic Syndrome, Type 2